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The experiment was conducted at the Research farm of Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, from February 2014 to June 2014. BARI Til-3 variety developed by Bangladesh Agricultural Research Institute (BARI), Gazipur. This experiment consisted of two factors; Factor-A: four levels of nitrogen viz. (i) N0 (Control), (ii) N1 (80 kg ha-1), (iii) N2 (100 kg ha-1) and (iv) N3 (120 kg ha-1) and Factor B: four levels of phosphorus viz. (i) P0 (Control), (ii) P1 (20 kg ha-1), (iii) P2 (30 kg ha-1) and (iv) P3 (40 kg ha-1). The experiment was laid out in a Randomized Complete Block Design (RCBD) with three replications. Data were recorded on different parameters such as the number of capsules plant-1, number of seeds capsule-1, the weight of 1000 seeds, seed yield ha-1 and stover yield ha-1. The study reveals that a combination of different levels of nitrogen and phosphorous have a significant influence on the yield of sesame. Highest seed yield ha-1 of sesame (1652 kg) and highest harvest index of sesame (37.33%) were recorded from N3P2 (120 kg N ha-1with 30 kg P2O5 ha-1). Therefore, the results suggest that the combined use of 120 kg N ha-1 and 30 kg P2O5ha-1 gave the highest yield of sesame.