Plug Cell Volume, Growing Media Quality and Benzyl Aminopurine (BAP) Spray Effects for Nursery Growth of Impatiens walleriana

Main Article Content

J. De Lojo
E. Gandolfo
E. Giardina
C. Boschi
A. Di Benedetto


Plant propagators must take two technological critical decisions: the plug-cell size and the growing medium, both of which have been mentioned as abiotic stress sources for bedding pot plants. However, only a few recent reports on bedding pot plants have simultaneously included limiting and non-limiting plug cell volumes and growing medium during nursery. The aim of this work was to assess the nursery performance of Impatiens walleriana seedlings grown in four plug cell volumes and four growing media with significant differences in both physical and chemical properties. Plants were sprayed or not with an early and single benzyl aminopurine (BAP) dose, aiming to understand how they interact on determining biomass accumulation at the pot transplant stage. The hypothesis tested was that, both plug cell volume and growing medium, must be seen as additive abiotic stress sources, which can be partially overridden by exogenous cytokinin supply. The main result was that, in I. walleriana seedlings, the abiotic stress imposed by the growing medium quality during nursery had a higher effect on biomass accumulation (on both fresh and dry base), leaf area expansion and photo assimilates partitioning than plug cell volume and constitute an interactive process associated with cytokinin synthesis. From a grower´s point of view, one expensive option to avoid root restriction is to use high quality growing media and increase the plug cell volume. In contrast, a single 100 mg L-1 BAP spray can partially override the root restriction symptoms related to abiotic stresses. The novelty of this work is related to the fact that growing media quality would be a more limited factor than plug cell volume for I. walleriana seedlings during nursery.

Abiotic stress, bedding plants, cytokinin, plug trays

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How to Cite
Lojo, J. D., Gandolfo, E., Giardina, E., Boschi, C., & Benedetto, A. D. (2019). Plug Cell Volume, Growing Media Quality and Benzyl Aminopurine (BAP) Spray Effects for Nursery Growth of Impatiens walleriana. International Journal of Plant & Soil Science, 27(6), 1-13.
Original Research Article


Di Benedetto A, Klasman R. The effect of plug cell volume on the post-transplant growth for Impatiens walleriana pot plant. Eur. J. Hort. Sci. 2004;69(2):82-86.

Di Benedetto A, Klasman R. The effect of plug cell volume, paclobutrazol height control and the transplant stage on the post-transplant growth for Impatiens wallerana pot plant. Eur. J. Hort. Sci. 2007;72(5):193-197.

Di Benedetto A. Root restriction and post-transplant effects for bedding pot plants. In: Ornamental Plants: Types, Cultivation and Nutrition. (J.C. Aquino, ed.), Nova Science Publishers, Inc. NY, USA. 2011;47-79.

Thibaud J, Mc. Loughlin T, Pagani A, Lavado R, Di Benedetto A. Alternative substrates and fertilization routine relationships for bedding pot plants: Impatiens wallerana. Eur. J. Hort. Sci. 2012;77(4):182-191.

Pagani A, Molinari J, Lavado RS, Di Benedetto A. Behavior of Impatiens wallerana Hook. F in alternative pot substrates: mechanisms involved and research perspectives. J. Plant Nutr. 2015;38:2185-2203.

Gandolfo E, Hakim E, Geraci J, Feuring V, Giardina E, Di Benedetto A. Responses of pansy (Viola wittrockiana Gams.) to the quality of the growing media. Amer. J. Exp. Agric. 2016;12(3):1-10.

Hakim G, Gandolfo E, Giardina E, Di Benedetto A.The effect of the pre-transplant pot media quality on pansy garden performance. Int. J. Plant Soil Sci. 2017;19(4):1-12.

Di Benedetto A, Pagani A. Difficulties and possibilities of alternative substrates for ornamental bedding plants: An ecophysiological approach. In: Draguhn C, Ciarimboli N, editors. Peat: Formation, Uses and Biological Effects, Nova Science Publishers, Inc. NY, USA; 2012.

DeLojo J, Gandolfo E, Gómez D, Feuring V, Monti S, Giardina E, Boschi C, Di Benedetto A. Root restriction effects on the bedding pot plant Impatiens walleriana. J. Exp. Agric. Int. 2017;15(4):1-16.

Gandolfo E, De Lojo E, Gómez E, Pagani A, Molinari J, Di Benedetto A. Anatomical changes involved in the response of Impatiens wallerana to different pre-transplant plug cell volumes and BAP sprays. Eur. J. Hort. Sci. 2014;79(4):226-232.

Di Matteo J, Rattin J, Di Benedetto A. Increase of spinach growth through the use of larger plug cell volume and an exogenous BAP spray. Amer. J. Exp. Agric. 2015;6(6):372-383.

Rattin J, Pico Estrada O, Giardina E, Di Benedetto A. Nursery pre- and post-transplant effects on tomato (Solanum lycopersicum L.) growth and yield. J. Exp. Agric. Int. 2017;18(5):1-14.

Geraci J, Di Matteo J, Feuring V, Giardina E, Di Benedetto A. Exogenous BAP spray on biomass accumulation in spinach. J. Exp. Agric. Int. 2018;25(6):1- 17.

Di Benedetto A, Klasman R, Boschi C. Use of river waste in growing media for growing ornamental herbaceous perennials. J. Hort. Sci. Biotechnol. 2004;79(1):119-124.

Marchese N, Di Benedetto A, Lavado R. The possibilities of river waste and Argentinean peat as a plug growing media for Verbena x hybrida. Int. J. Agric. Res. 2006;1(2):142-150.

Di Benedetto A, Petracchi JC, Marcella G, Montaron P, Chavez P. Evaluation of alternative substrates for bedding plants. Int. J. Agric. Res. 2006;1(6):545-554.

Pagani A, Molinari J, Lavado RS, Di Benedetto A. Behavior of Impatiens wallerana Hook. F in alternative pot substrates: mechanisms involved and research perspectives. Journal of Plant Nutr. 2015;38:2185-2203.

Ghanem ME, Albacete A, Smigocki AC, Frebort I, Pospilova H, Martinez-Andujar C, Acosta M, Sanchez-Bravo J, Lutts S, Dodd IC, Perez-Alfocea F. Root synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants. J. Exp. Bot. 2011; 6(1):125-140.

Hwang I, Sheen J, Müller B. Cytokinin signaling networks. Annual Rev. Plant Biol. 2012;63:353-80.

Brenner WG, Schmülling T. Summarizing and exploring data of a decade of cytokinin-related transcriptomics. Front. Plant Sci. 2015;6:29.

O’Hare TJ, Turnbull CGN. Root growth, cytokinin and shoot dormancy in lychee (Litchi chinensis Sonn.). Scientia Hortic. 2004;102(2):257-266.

Aloni R, Langhans M, Aloni E, Dreieicher E, Ullrich CI. Root-synthesized cytokinin in Arabidopsis is distributed in the shoot by the transpiration stream. J. Exp. Bot. 2005; 56(416):1535-1544.

Kotov AA, Kotova LM. Role of acropetal water transport in regulation of cytokinin levels in stems of pea seedlings. Russian J. Plant Physiol. 2015;62(3):390-400.

Williams T, Sala A, Feuring V, Giardina E, Di Benedetto A. Relationships between plug cell size and quality substrate in the bedding pot plant Impatiens wallerana (Hook.f.). Int. J. Plant Soil Sci. 2016;12(2): 1-12.

Faust JE, Holcombe V, Rajapakse NC, Layne DR. The effect of daily light integral on bedding plant growth and flowering. Hort Science. 2005;40(3):645-649.

Pramuk LA, Runkle ES. Modeling growth and development of Celosia and Impatiens in response to temperature and photosynthetic daily light integral. J. Amer. Soc. Hort. Sci. 2005;130(6):813-818.

Henson DY, Newman SE, Hartley DE. Performance of selected herbaceous annual ornamentals grown at decreasing levels of irrigation. Hort Science. 2006; 41(6):1481-1486.

Di Benedetto A, Pagani A. Dry weight accumulation in the Impatiens walleriana pot plant in responses to different pretransplant plug cell volume. Europ. J. Hort. Sci. 2013;78(2):76-85.

Fonteno WC. Growing Media Types and Physical/Chemical Properties. In: Water, Media and Nutrition for Greenhouse Crops. A Grower´s Guide. (Reed, D.W., Ed.), Ball Publishing, Batavia, Illinois, U.S.A.; 1996.

Potter J, Jones JW. Leaf area partitioning as an important factor in growth. Plant Physiol. 1977;59(1):10-14.

Warton DI, Duursma RA, Falster DS, Taskinen S. SMATR 3-an R package for estimation and inference about allometric lines. Methods Ecol. Evol. 2012;3(2):257-259.

Cramer GR, Urano K, Delrot S, Pezzptti M, Shinozaki K. Effects of abiotic stress on plants: A systems biology perspective. BMC Plant Biol. 2011;11(1):163-176.

Ghosh D, Xu J. Abiotic stress responses in plant roots: A proteomics perspective. Front. Plant Sci. 2014;5:6.

Shi K, Ding XT, Dong DK, Zhou YH, Yu JQ. Root restriction-induced limitation to photosynthesis in tomato (Lycopersicon esculentum Mill.) leaves. Scientia Hortic. 2008;117(3):197-202.

Shi K, Fu LJ, Dong DK, Zhou YH, Yu JQ. Decreased energy synthesis is partially compensated by a switch to sucrose synthase pathway of sucrose degradation in restricted root of tomato plants. Plant Physiol. Biochem. 2008;46(12):1040-1044.

Poorter H, Bühler J, van Dusschoten D, Climent J, Postma JA. Pot size matters: A meta-analysis of the effects of rooting volume on plant growth. Funct. Plant Biol. 2012;39(11):839-850.

Puig J, Pauluzzi G, Guiderdoni E, Gantet P. Regulation of shoot and root development through mutual signaling. Mol. Plant. 2012;5(5):974-983.

Chen BJ, During HJ, Vermeulen PJ, Kroon H, Poorter H, Anten NP. Corrections for rooting volume and plant size reveal negative effects of neighbour presence on root allocation in pea. Functional Ecol. 2015;29(11):1383-1391.

Coro M, Araki A, Rattin J, Miravé P, Di Benedetto A. Lettuce and celeryresponses to both BAP and PBZ related tothe plug cell volume. Amer. J. Exp. Agric. 2014;4(10):1103-1119.

Yruela I. Plant development regulation: Overview and perspectives. J. Plant Physiol. 2015;182(5): 62-78.

Rattin J, Wagner P, Ferreyro D, Riverti D, Giardina E, Di Benedetto A. Roots partially drive super sweet maize yield. J Exp. Agric. Int. 2017;16(6):1-17.

Lee BH, Johnston R, Yang Y, Gallavotti A, Kojima M, Travencolo BAN, Costa LF, Sakakibara H, Jackson D. Studies of aberrant phyllotaxy1 mutants of maize indicate complex interactions between auxin and cytokinin signaling in the shoot apical meristem. Plant Physiol. 2009; 150(1):205-216.

Shani E, Ben-Gera H, Shleizer-Burko S, Burko Y, Weiss D, Ori N. Cytokinin regulates compound leaf development in tomato. Plant Cell. 2010;22(10):3206-3217.

Francis D, Halford NG. Nutrient sensing in plant meristems. Plant Mol. Biol. 2006; 60(6):981-993.

Enquist BJ, Kerkhoff AJ, Stark SC, Swenson NG, McCarthy MC, Price CA. A general integrative model for scaling plant growth, carbon flux, and functional trait spectra. Nature. 2007;449(7159):218-222.

Demura T, Ye ZH. Regulation of plant biomass production. Curr. Op. Plant Biol. 2010;13(3):298-303.

Shipley B. Net assimilation rate, specific leaf area and leaf mass ratio: Which is most closely correlated with relative growth rate? A meta-analysis. Funct. Ecol. 2006; 20(4):565-574.

Boonman A, Prinsen E, Gilmer F, Schurr U, Peeters AJ, Voesenek LA, Pons TL. Cytokinin import rate as a signal for photosynthetic acclimation to canopy light gradients. Plant Physiol. 2007;143(4): 1841-1852.

Wu X, Zhu Z, Li X, Zha D. Effects of cytokinin on photosynthetic gas exchange, chlorophyll fluorescence parameters and antioxidative system in seedlings of eggplant (Solanum melongena L.) under salinity stress. Acta Physiol. Plant. 2012; 34(416):2105-2114.

Van Staden J, Zazimalova E, George EF. Plant growth regulators II: Cytokinins, their analogues and antagonists. In: George EF, Hall MA, De Klerk GJ, Editors. Plant Propagation by Tissue Culture Springer. The Netherlands; 2008.

Poorter H, Sack L. Pitfalls and possibilities in the analysis of biomass allocation patterns in plants. Front. Plant Sci. 2012; 3:259.

Feller C, Favre P, Janka A, Zeeman SC, Gabriel JP, Reinhardt D. Mathematical modeling of the dynamics of shoot-root interactions and resource partitioning in plant growth. PloS One. 2015;10: e0127905.

Benincasa P, Guiducci M, Tei F. The nitrogen use efficiency: meaning and sources of variation-case studies on three vegetable crops in central Italy. Horttechnol. 2011;21(3):266-273.

Kobe RK, Iyer M, Walters MB. Optimal partitioning theory revisited: Nonstructural carbohydrates dominate root mass responses to nitrogen. Ecology. 2010; 91(1):166-179.

Yang Z, Midmore DJ. Modelling plant resource allocation and growth partitioning in response to environmental heterogeneity. Ecol. Model. 2005;181(1): 59-77.

Price CA, Weitz JS. Zero-sum allocational strategies determine the allometry of specific leaf area. Am. J. Bot. 2010; 97(11):1808-1815.

Zwack PJ, Rashotte AM. Interactions between cytokinin signalling and abiotic stress responses. J. Exp. Bot. 2015; 66(16):4863-4871.