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Application of undecomposed green manure has been reported to cause poor emergence and establishment of common beans in the field. Therefore, to understand the mechanisms’ contributing to the poor crop establishment, the effect of extracts from fresh and decomposed legume green manures on bean seed germination, fungal mycelial growth, spore germination and germ tube elongation were evaluated. The extracts were prepared in either ethanol or distilled water. Data was collected on percentage seed germination, seedling length, mycelial radial growth, spore germination and germ tube elongation. Ethanol extracts from fresh lablab inhibited bean germination by 56%, increased mean germination time to 8 days, and decreased germination index while ethanol extracts of groundnut and beans caused highest inhibition in bean shoot length and reduced biomass. Ethanol extracts from fresh green manures significantly inhibited fungal mycelial growth while the aqueous extracts from beans, groundnuts and soybean had significant level of antifungal activity while aqueous lablab extracts stimulated mycelial. Aqueous extract of lablab and soybean enhanced spore germination by over 70% with more pronounced effect on germ tube length and number of germ tubes by 8.0% and 13% respectively. The study comparatively reveals that the extract of lablab was inhibitory to common bean germination compared to other legume extracts and also stimulated the growth of root rot pathogens that may have resulted in poor establishment of beans.
Kaur S, Singh HP, Batish DR, Kohli RK. Phytotoxicity of decomposing below-ground residues of Ageratum conyzoides: Nature and dynamics of release of phytotoxins. Acta Physiologiae Plantarum. 2012; 34(3):1075-1081.
Jilani G, Mahmood S, Chaudhry AN, Hassan I, Akram M. Allelochemicals: Sources, toxicity and microbial transformation in soil- A review. Annals Microbiol. 2008;58(3):351-357.
Farooq M, Bajwa AA, Cheema SA, Cheema ZA. Application of allelopathy in crop production. Int. J. Agric. Biol. 2013; 15:1367–1378.
Cheng F, Cheng Z. Research progress on the use of plant allelopathy in agriculture and the physiological and ecological mechanisms of allelopathy. Frontiers Plant Sci. 2015; 6:1020-1018.
Bonanomi G, Cesarano G, Lombardi, N, Motti R, Scala F, Mazzoleni S, Incerti G. Litter chemistry explains contrasting feeding preferences of bacteria, fungi, and higher plants. Sci reports. 2017;7(1):1-13.
Herro JL, Callaway RM. Allelopathy and exotic plant invasion. Plant and Soil. 2003; 256:29-39.
Ayub M, Ijaz MK, Tariq M, Tahir M, Nadeem MA. Allelopathic effects of winter legumes on germination and seedling indicators of various summer cereals. Agric Trop Subtrop. 2012;45:179-183.
Lertmongkol, Sarobol ED, Premasthira C. Allelopathic effects of mung bean (Vigna radiata L.) on subsequent crops. Kasetsart J. Nat Sci. 2011;45:773-779.
Al-Mughrabi, K. Antimicrobial activity of extracts from leaves, stems and flowers of Euphorbia macroclada against plant pathogenic fungi. Phyto Med. 2003;42(3): 245-250. DOI:10.14601/Phytopathol_Mediterr-1712.
Dhole JA, Bodke S, Dhole N. Allelopathic effect of aqueous extract of five selected weed species on seed mycoflora, seed germination and seedling growth of Sorghum vulgare Pers. Research J. Pharm Bio Chem Sci. 2011;2(3):142-8.
Chukwuka KS, Obiakara MC, Ogunsumi IA. 2014. Effects of aqueous plant extracts and inorganic fertilizer on the germination, growth and development of maize (Zea mays L.). J. Agric Sci. 2015;59(3):243- 254.
Afzal MI, Iqbal MA, Cheema ZA. Triggering growth and boosting economic yield of late-sown wheat (Triticum aestivum L.) with foliar application of allelopathic water extracts. World J. Agric Sci. 2015;11:94-100.
Jules CN, Assob-Henri LF, Kamga Dickson S, Nsagha, Anna L Njunda, Peter F Nde, Emmanuel A Asongalem, Abdel J Njouendou, Bertrand Sandjon, Veronique B Penlap. Antimicrobial and toxicological activities of five medicinal plant species from Cameroon. Traditional Medicine. BMC Comp Alt Med. 2011;11:70. DOI:10.1186/1472-6882-11-70.
Ingham ER. Compost tea brewing manual. 3rd Edition. 2002;78.
Cieniak C, Walshe-Roussel B, Liu R, Muhammad A, Saleem A, Haddad PS, Cuerrier A, Foster BC Arnason JT. Phytochemical comparison of the water and ethanol leaf extracts of the Cree medicinal plant, Sarracenia purpurea L.(Sarraceniaceae). J. Pharm and Pharmaceut Sci. 2015;18:484-493.
Hadi M, Kashefi B, Sobhanipur A, Rezaarabsorkhi M. Study on effect of some medicinal plant extracts on growth and spore germination of Fusarium oxysporum schlecht. in vitro. American-Eurasian J. Agric and Environ Sci. 2013; 13(4): 581-588.
Oliver OO, James WM, John O, Rama N, John HN. Effect of lablab green manure on population of soil microorganisms and establishment of common bean (Phaseolus vulgaris L.). American J. Agric Sci. 2018; 5(3): 44-54.
Dezfuli PM, Sharif-Zadeh F, Janmohammadi M. Influence of priming techniques on seed germination behavior of maize inbred lines (Zea mays L.). J. Agric and Bio Sci. 2008; 3(3): 22-25.
Nollet LM, Rathore HS. Handbook of pesticides: Methods of pesticides residue analysis. USA: Taylor and Francis. 2010; 608.
Amadi JE, Adeleke EE, Olahan G, Garuba T, Adebola, MO. Effect of plant extracts on sporulation and spore germination of stored melon seed fungi. Intern J. Res-Granthaalayah. 2014;1(1):21-29.
Djenane Z, Nateche F, Amziane M, Gomis-Cebolla J, El-Aichar F, Khorf H, Ferré J. Assessment of the antimicrobial activity and the entomocidal potential of Bacillus thuringiensis isolates from Algeria. Toxins. 2017; 9(4):139.
Masoud EA, Gouda, HA. Effect of some natural plant extracts against gram negative bacteria in Njran Area, Saudi Arabia. Egyptian Academy Journals of Biological Sciences. 2012; 4: 85-92.
Rahman MA, Begum MF, Alam MF. Screening of Trichoderma isolates as a biological control agent against Ceratocystis paradoxa causing pineapple disease of sugarcane. Mycobio. 2009; 37(4):277-285.
Lawes Agricultural Trust, Harpenden. Guide to the classical experiments. Rothamsted Experimental Station. UK; 1991.
Onivogui G, Letsididi R, Diaby M, Wang L, Song Y. Influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R. Br. ex Sabine fruits. Asian Pacific J. Trop Biomed. 2016; 6(1):20-25.
Kato-Noguchi H, Mominul AKM Islam. Phytotoxic activity of Ocimum tenuiflorum extracts on germination and seedling growth of different plant species. The Scient World J; 2014.
Hussain MI, Gonzalez-Rodriguez L, Reigosa MJ. Germination and growth response of four plant species to different allelochemicals and herbicides. Allelopathy J. 2008;22:101–110.
Soltys D, Bogatek H, Gniazdowskas A. Phytotoxic effects of cyanamide on seed germination and seedling growth of weed and crop species, Acta Biologica Cracoviensia. 2012; 54:87–92.
Haramoto ER, Gallandt, ER. Brassica cover cropping: II. Effects on growth and interference of green bean (Phaseolus vulgaris) and redroot pigweed (Amaranthus retroflexus). Weed Sci. 2005; 53:702-708.
Bonanomi G, Incerti G, Barile E, Capodilupo M, Antignani V, Mingo A, Mazzoleni S. Phytotoxicity, not nitrogen immobilization, explains plant litter inhibitory effects: evidence from solid‐state 13C NMR spectroscopy. New Phytologist. 2011;191:1018-1030.
Shankar MR, Veeralakshmi S, Sirajunnisa AR, Rajendran R. Effect of allelochemicals from leaf leachates of Gmelinaarborea on inhibition of some essential seed germination enzymes in green gram, red gram, black gram, and chickpea. Intern scholarly res notices; 2014.
Article ID 108682,
Terzi I. Allelopathic effects of Juglone and decomposed walnut leaf juice on muskmelon and cucumber seed germination and seedling growth. Afr J. Biotech. 2008;7:1870–1874.
Matthews S, Khajeh HM. Length of the lag period of germination and metabolic repair explain vigour differences in seed lots of maize (Zea mays). Seed Sci and Tech. 2007;35: 200-212.
Demir I, Ermis S, Okcu G, Matthews S. Vigour tests for predicting seedling emergence of aubergine (Solanum melongena L.) seed lots. Seed Sci and Tech. 2005;33:481-484.
Tanveer A, Safdar ME, Tariq MA, Yasin M, Noorka IR. Allelopathic inhibition of germination and seedling vigor of some selected crops by Achyranthes aspera L. Herbologia. 2014;14:35-46.
Pawar KB, Chavan PD. Influence of leaf leachates of soybean, Moringa, Parthenium and Eucalyptus on carbohydrate metabolism in germinating seeds of Sorghum bicolor (L.) Moench. Allelopathy J. 2007;19: 543–548.
Mohammadi Z, Atik F. Fungitoxic effect of natural extracts on mycelial growth, spore germination and aflatoxin B1 production of Aspergillus flavus. Australian J. Crop Sci. 2013;7(3):293- 298.
Arif T, Bhosale JD, Kumar N, Mandala TK, Bendre RS, Lavekar GS, Dabura R. Natural products – antifungal agents derived from plants. J. Asian Natural Products Res. 2009;11: 621-638.
Balekari U. Antihyperglycemic and antihyperlipidaemic activities of Dolichos Lablab (Lablab purpureus) seed extract on streptozotocin - nicotinamide induced diabetic rats. International Conference and Exhibition on Pharmacognosy, Phytochemistry and Natural Products, Hyderabad- India. 2013;21-23.
Okigbo RN, Odurukwe CN. Occurrence and control of fungal rot pathogens of yams (Dioscoreaspp) with leaf extracts of Chromolena odorata, Carica papaya and Aspilia africana. Nigerian Journal of Mycology. 2009; 2(1):154-165.
Shojaee M, Sadeghi-Mahoonak AR, Khomeiri M, Ghorbani M. Antibacterial Effects of Stevia rebaudianaBertoni Extract on Pathogenic Bacteria and Food Spoilage. Journal of Food Biosciences and Technology .2017;7(1):57-64.
Torres RC, Manalo JB. Phyto-chemical investigation of Dolichos lablab L. by thin layer chromatography. Philippine Techn J. 1990;15(1):41-50.