A Comparative Evaluation of Selected Medicinal Plants on Male Fertility Indices (Reproductive Hormones and Sperm Profile) of Albino Wistar Rats: An Animal Case Study

Main Article Content

Oliver E. Ngwu
J. I. Okoye

Abstract

The study aims to investigate the effects of the medicinal plants {seeds} on the  reproductive hormones and sperm profile of male albino rats to ascertain their possible usefulness  as  fertility agent. Walnuts [Tetracarpidium conophorum], Sesame (Sesamine indicum), and Velvet bean (Mucuna pruriens) seeds were obtained and taken to the Department of plant  Science and  Biotechnology, University of  Nigeria, Nsukka (UNN). The seeds were milled into fine powder. One hundred and ten sexually matured aibino rats of about twelve weeks weighing 130-180 g were divided into eleven groups (1-11) using completely randomized design. There were two different control groups and rats in group 1 served as the Control 1 and were fed with normal commercial feed. Rats in group 2 were administered with a drug (Ketoconazole) to induce infertility. The Infertility Induced groups were treated with low dose (groups 3-5), medium dose (groups 6-8) and  high dose (9-11) for the period of nine (9) weeks. At the end and testes and Epididymides were surgically removed and weighed. Blood  sample analysis revealed that the concentration of sex hormones  measured in the male rats fed with medium plants (seeds) showed that the testosterone concentration significanty increased (p<0.05) in animal control group 1(normal rats) Therefore,  these medicinal plants walnut seeds showed significant increase in their testosterone concentration, luteinizing hormone (LH) and follicle stimulating hormone (FSH) which significantly enhanced the production of reproductive hormones which enriched the fertility status of these animals.

Keywords:
Albino rats, medicinal plants, reproductive hormones, sperm profile, testis histology

Article Details

How to Cite
E. Ngwu, O., & Okoye, J. I. (2019). A Comparative Evaluation of Selected Medicinal Plants on Male Fertility Indices (Reproductive Hormones and Sperm Profile) of Albino Wistar Rats: An Animal Case Study. International Journal of Plant & Soil Science, 29(2), 1-6. https://doi.org/10.9734/ijpss/2019/v29i230138
Section
Original Research Article

References

Golla UR, Gajam PK, Mohammad AR, Kumar AK, Raj BSS. Assessment of bioactivity of Desmostachya bipinnata (L.) Stapf using brine shrimp (Artemia salina) lethality assay. Pharmacologyonline. 2011; 3:982-990.

Krishnaraju AV, Rao TV, Sundararaju D, Vanisree M, Tsay HS, Subbaraju GV. Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay. Int J Appl Sci Eng. 2005;3(2):125-34.

Mukherjee PK, Wahile A. Integrated approaches towards drug development from Ayurveda and other Indian system of medicines. Journal of Ethnopharmacology. 2006;103(1):25-35.

AJaiyeoba EO, Fadare DA. Antimicrobial potential of extracts and fractions of the African Walnut Tetracarpidium conophorium. African Journal of Biotechnology. 2006;5(22):2322-2325.

Orth AB, Royse DJ, Tien M. Ubiquity of lignin–degrading peroxidases among various wood-degrading fungi. Applied Environmental Microbiology. 1993;59: 4017-4023.

Dashistani HI, Dayem MA. Hyper-prolactinemia and hyper gonadotro-pins in infertile men with severe oligospermia and azospermia. International Journal of Endocrinology. 2007;3:3-8.

Zwarts L, Savage GP, Mc Neil BL. Fatty acid content of new Zealand-grown walnuts (Juglans regia L). International Journal of Food Sciences and Nutrition. 1999;50:189.

Vawda AI, Davies AG. An investigation into the effects of Ketoconazole on testicular function in wistar rats. Acta Endocrinology. 1986;111(2):246-251.

Ekaluo UB, Ikpeme EV, Udensi O, Markson AA, Madunagu BE, Omosun G, Umana E. Effect of aqueous leaf extract of neem (Azadijirachta indica) on the hormonal milieu of male rats. International Journal of Current Research; 2010.

World Health Organization. WHO laboratory manual for the Examination of human Semen and Sperm. Cervical Mucus Interaction Cambridge University Press; 1992.

Bjorndahl L, Sodourlund I, Kvist U. Evaluation of the one–step eosin- nigrosin staining technique for human sperm vitality assessment. Human Reproduction. 2003;18:813- 816.

Ekaluo UB, Udokpoh AE, Udofia UU, Ajang RO. Comparative toxicity of five commonly used analysis, on rat sperm Count and Morphology. Global Journal of Pure and Applied Sciences. 2005;2(1):81-82.