Effect of Plant Growth Regulators (BA, KIN and NAA) on In vitro Propagation of Papaya (Carica papaya)

Main Article Content

Nahida Hasan
Humayra Huq
Fahima Khatun
Shamim Ara Sumi

Abstract

The present research was carried out in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207 from the period of September 2017 to June 2018. This research aims to study the effect of Benzyladenine (BA), Kinetin (KIN) and Naphthalene acetic acid (NAA) either in combination or alone on In vitro propagation of papaya (Carica papaya). The shoot tips of young shoots were used as explant, which was sterilized using freshly prepared 0.1% HgCl2 mixing with few drops of Tween-20, were inoculated in MS media supplemented with 0.1% activated charcoal. The minimum days to shoot induction (10.25) were recorded on MS medium containing 0.5 mg/L BA. The highest shoots (4.5) and length of shoot (5.75 cm) observed in 1.0 mg/L BA. The combined treatment 1.0 mg/L BA+0.75 mg/LKIN gave the highest number of shoots (5.25) and length of shoot (5.78 cm).The minimum days (8.5) to root induction was reported in 2.0 mg/L NAA along with maximum 8.25 roots per plantlet. The highest length of root (6.92 cm) was observed in 2.0 mg/L NAA. In regenerated plantlets, 80% survival rates were observed in growth chamber conditions and 75% in the open atmosphere were achieved. Finally, the in vitro regeneration protocol described herein can potentially be used as a tool in molecular breeding programs for the improvement of different cultivars and genotypes of papaya.

Keywords:
BA, KIN, NAA, explants, Carica papaya

Article Details

How to Cite
Hasan, N., Huq, H., Khatun, F., & Sumi, S. A. (2020). Effect of Plant Growth Regulators (BA, KIN and NAA) on In vitro Propagation of Papaya (Carica papaya). International Journal of Plant & Soil Science, 32(5), 15-23. https://doi.org/10.9734/ijpss/2020/v32i530278
Section
Original Research Article

References

Badillo VM. Caricaceae. Segundo Esquema. Revista de la Facultad de Agronomia De la Universidad Central de Venezuela, Maracacy, Venezuela. 1993;43:1-111.

Canini A, Alesiani D, D’Arcangelo G, Tagliatesta P. Gas chromatography-mass spectrometry analysis of phenolic compounds from Carica papaya L. leaf. J Food Composit Anal. 2007;20(7):584- 590.

Seelig RA. Papaya. Fruit and vegetable facts and pointers. United Fresh Fruit and Vegetable Association, Washington, DC; 1970.

Nakasone HY, Paull RE. Tropical fruits. CAB International, Wallingford; 1998.

Malabadi RB, Kumar SV, Mulgund GS, Nataraja K. Induction of somatic embryogenesis in papaya (Carica papaya). Research in Biotechnology. 2011;2(5):40-55.

Bhattacharya J, Khuspe SS. In vitro and in vivo germination of papaya (Carica papaya L.) seeds. Scientia Horticulturae. 2001;91(1&2):39-49.

Maushardt RM. ‘Papaya’ in biotechnology of perennial fruit crops. F. A. Hamonerschlagand, R. E. Litz, Eds. CABI, London. 1992;489-511.

Purnima S, Sandhya B. Genotypic difference of in vitro lateral bud establishment and shoot proliferation in papaya. Biot. Cent. In: Indian Agric Res. Ins.; 1988.

FAOSTAT. Food and agriculture organisation for the United Nations –Statistics. Online Database; 2017.
Available:http://faostat.fao.org

Tsai SF, Yeh SD, Chan CF, Liaw SI. High-efficiency vitrification protocols for cryopreservation of in vitro grown shoot tips of transgenic papaya lines. Plant Cell, Tissue and Organ Culture. 2000;98:157-164.

Rajeevan MS, Pandey RM. Lateral bud culture of papaya (Carica papaya L.) for clonal propagation. Plant Cell, Tissue and Organ Culture. 1986;5:181-188.

Litz RE, Conover RA. High frequency somatic embryogenesis from Carica suspension cultures. Ann Bot. 1983;51: 683-686.

Saker MM, Bekheet SA, Taha HS, Reda AA. In vitro propagation of papaya (Carica papaya L.). Science and Biotechnology. 1999;1:47-43.

Chan LK, Teo CKH. Micropropagation of Eksotika, a Malaysia papaya cultivar, and the field performance of the tissue culture derived clones. Acta Hort. 2002;575:99-105.

Yanmaz R, Yazar E, Kantoglu KY, Alper A. In vitro plant regeneration and bulblet formation of Tunceli garlic (Allium tuncelinum) (Kollman) by shoot and root culture. J. Food, Agri. & Env. 2010;3&4: 572-576.

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962;15:473-97.

Bhandari AK, Negi J, Bisht VK, Bharti MK. In vitro propaation of Aloe vera- A plant with medicinal properties. Nature and Science. 2010;8(8):174-176.

Gantait S, Mandal N, Bhattacharya S, Das PK. A novel strategy for in vitro conservation of Aloe vera L. through long term shoot culture. Biotechnol. 2010;9:218-223.

Baksha R, Jahan MAA, Khatun R, Munshi JL. Micropropagation of Aloe barbadensis Mill. through in vitro culture of shoot tip explants. Plant Tiss. Cult. & Biotechnol. 2005;15(2):121-126.

Petkova S, Popova N, Angelova Y, Stefanov B, Iliev L, Popov M. Inhibitory effect of some plant growth regulators and chlorosulfuron on growth, protein composition and proteolytic activity of maize seedlings. Biotechnol and Biotechnol. 2003;17(2):77-83.

Islam M. In vitro plant regeneration and improvement of Carica papaya L. through somaclonal variation. Ph.D. Thesis. Plant Breeding and Biotechnology Laboratory, Department of Botany, University of Rajshahi, Bangladesh; 2001.

Islam R, Rahman SM, Hossain M, Joarder OI. In vitro clonal propagation of papaya through culture of lateral buds from mature field-grown plants. Plant Tissue Cult. 1993;3:47-50.

Chowdhury AR. Propagation of papaya through tissue culture. Ann. Bangladesh Agric. 1991;1:45-46.