Rapid Methods for Identification of Plant-Growth-Promoting Rhizobacteria
Sangita Sahni
Department of Plant Pathology, TCA, Dholi, Dr. Rajendra Prasad Central Agricultural University, Pusa, Bihar, India.
Bishun Deo Prasad
*
Department of Molecular Biology and Genetic Engineering, Bihar Agricultural College, Sabour, BAU, Sabour, Bhagalpur, Bihar, India.
*Author to whom correspondence should be addressed.
Abstract
The growing human population has put enormous pressure on agriculture to increase production and productivity, which has resulted in the widespread usage of agrochemicals. The indiscriminate use of agrochemicals has harmed soil fertility and resulted in significant environmental contamination, impacting human health. The discovery and implementation of bacterial isolates with Plant Growth Promoting Rhizobacteria (PGPR) have enormous potential for reducing the usage of chemical fertilizers, insecticides, and herbicides. However, the identification of bacterial isolates is a prerequisite to utilize them for agricultural purposes. Traditional methods involve culturing microbes using a range of nonselective and selective enrichment methods, followed by biochemical confirmation among others. Traditional methods involve culturing microbes, followed by morphological biochemical confirmation etc. In the present investigation, we describe a fast and effective approach for isolating and identifying bacterial isolates, followed by phylogenetic analysis and submission to GenBank.
Keywords: Plant Growth Promoting Rhizobacteria (PGPR), 16S rDNA, Gram staining, Phylogenetic analysis, GenBank