Exogenously Applied Proline Modulates Drought Tolerance in Maize (Zea mays L)

Shamim Akram

Soil Science Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202, Bangladesh.

Mohammad Golam Kibria

Department of Soil Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Yoshiyuki Murata

Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.

Md. Anamul Hoque *

Department of Soil Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

*Author to whom correspondence should be addressed.


Abstract

Improving drought stress tolerance in maize is essential to increase its production and yield worldwide.  Thus, the present study was conducted to investigate the improvement of drought tolerance in maize (Zea mays L.) by exogenous application of proline (25 and 50mM) on two maize varieties. Maize plants were subjected to drought stress at various phases of plant growth under pot culture conditions and proline was applied as foliar spray. Water deficit stress caused a significant decrease (by approximately 25%) in growth and yield of both maize varieties by decreasing plant height, cob length, dry root weight, grains per cob and 100-grain weight. Water deficit stress also decreased chlorophyll and intercellular proline contents, and antioxidant enzyme activities viz. catalase (CAT), guaiacol peroxidase (POX) and ascorbate peroxidase (APX). Exogenous application of proline (50 mM) was found to be more effective in increasing growth and yield of both varieties. These increases were positively associated with increased levels (by at least 15%) of chlorophyll and intracellular proline, and enhanced activities of CAT, POX and APX enzymes in both varieties. Interaction effects of exogenous proline and water deficit stress were significant in aspects of higher growth and yields and enhanced levels of chlorophyll, intracellular proline and antioxidant enzyme activities. Therefore, it is concluded that foliar application of proline improves drought tolerance by modulating chlorophyll and intracellular proline contents, and antioxidant enzyme activities.

Keywords: Drought stress, proline, chlorophyll, antioxidant enzymes


How to Cite

Akram, Shamim, Mohammad Golam Kibria, Yoshiyuki Murata, and Md. Anamul Hoque. 2021. “Exogenously Applied Proline Modulates Drought Tolerance in Maize (Zea Mays L)”. International Journal of Plant & Soil Science 33 (19):198-212. https://doi.org/10.9734/ijpss/2021/v33i1930619.