Determination of Insecticidal Potential of Lentil (Lens culinaris L.) Lectin through Bio-computational Tools
Rakesh Kumar Prajapat *
School of Agriculture, Suresh Gyan Vihar University, Jaipur, India.
Pawan Mainkar
School of Agriculture, Suresh Gyan Vihar University, Jaipur, India.
Sarika Sahoo
ICAR- Indian Agricultural Statistics Research Institute, Pusa New Delhi-12, India.
RekhaKansal .
School of Agriculture, Suresh Gyan Vihar University, Jaipur, India.
*Author to whom correspondence should be addressed.
Abstract
Plant and animals are the chief source of lectins playing important role in defense system against various pathogens. Lentil (Lens culinaris) lectin (LL) binds to polysaccharides and other glycoconjugates in a reversibly manner which contain glucose or mannose type sugars. In present investigation, the insecticidal potential of LL is determined through In-silico study by using various bio-computational tools against mustard aphid. The physico-chemical property of LL also exhibited theoretical pI is 5.22, GRAVY value is -0.086, aliphatic index is 78.33 and instability index is 27.51. The functional domain exposure of LL showed metal interacting and N-linked glycosylated amino acid residues. The structural behavior and biological activity of LL protein is determined by secondary structure prediction and its other attributes. Present investigation explores the potential of insecticidal activity of lentil lectin against mustard aphid by using numerous In silico approaches. This probably helps in understanding of LL three dimensional protein structures.
Keywords: Legume lectins, functional domain, molecular docking, molecular modeling and Insecticidal potential