In silico Analysis of Transcription Factor Binding Sites and Impact of Defense Responsive Phytohormones in OsPR1a

Diksha Kumari

Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, UP, India.

Bishun Deo Prasad *

Department of Agricultural Biotechnology & Molecular Biology, College of Basic Sciences & Humanities, Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur, Bihar-848125, India.

Padmanabh Dwivedi

Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, UP, India.

Sangita Sahni

Department of Plant Pathology, TCA, Dholi, Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur, Bihar-848125, India.

*Author to whom correspondence should be addressed.


Abstract

A variety of biotic and abiotic stress conditions result in the production and accumulation of pathogenesis related (PR) proteins in plant. Transcriptional regulation of PR genes plays a vital role in defense response in plant. In rice, the role of the PR1 gene in defense response have been studied, but critical examination of the OsPR1a gene after a treatment with defense responsive phytohormones and their regulation via promoter analysis have not been examined indepth. Several signalling mechanisms are involved in the induction and repression of defense genes, which are mediated by salicylic acid (SA) and jasmonic acid (JA). Expression profiling was carried out to determine the effects of phytohormones, salicylic acid (SA), and jasmonic acid (JA) at 12 h, 24 h, and 48 h after treatment. Expression profiling indicates cumulative upregulation of OsPR1a gene at 12 h after SA and JA treatments, whereas it downregulates at 24 h and 48 h after JA treatment. We also performed a comprehensive in silico analysis of the promoter region of OsPR1a gene to predict how the transcription factor binding site (TFBS) regulate its expression.

Keywords: OsPR1a, salicylic acid (SA), jasmonic acid (JA), transcription factor binding sites (TFBSs)


How to Cite

Kumari, Diksha, Bishun Deo Prasad, Padmanabh Dwivedi, and Sangita Sahni. 2022. “In Silico Analysis of Transcription Factor Binding Sites and Impact of Defense Responsive Phytohormones in OsPR1a”. International Journal of Plant & Soil Science 34 (20):338-45. https://doi.org/10.9734/ijpss/2022/v34i2031161.