Morphological and Molecular Characterization of Colletotrichum gloeosporioides Causing Mango Anthracnose
M. Kaviyarasi
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
A. Kamalakannan *
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
L. Rajendran
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
S. Rajesh
Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
M. Kavino
Department of Horticulture, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
K. R. Swarna Lakshmi
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
J. Shajith Basha
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Mango (Mangifera indica), a fruit with high nutritional value is widely regarded as a most palatable fruit crop which is affected by a number of biological constraints mainly diseases. Anthracnose caused by Colletotrichum gloeosporioides belongs to order Melanconiales is the important post harvest disease which restricts the marketing of mango. In the present study, thirteen isolates obtained from various places of Tamil Nadu were collected and isolated by tissue segment approach and purified. The isolates were confirmed phenotypically using morphological characters. Molecular investigation like PCR assay using universal primers ITS1 and ITS4 produced amplicon size of 560bp. The isolates were also identified using genus specific and species specific primers which resulted in the amplicon size of 280bp and 380bp. As a result, The use of morphological and molecular approaches to characterise Mango anthracnose will be useful in identifying and managing the disease.
Keywords: Mangifera indica, Colletotrichum gloeosporioides, molecular characterization