Coat Protein Characterization of Mungbean yellow mosaic virus Infecting Blackgram (Vigna mungo L. Hepper) in Tamil Nadu
Nimmala Sree Valli
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore- 641003, India.
G. Karthikeyan *
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore- 641003, India.
S. Harish
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore- 641003, India.
M. Sudha
Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore-641003, India.
D. Jeya Sundara Sharmila
Department of Nano Science and Technology, Tamil Nadu Agricultural University, Coimbatore- 641003, India.
*Author to whom correspondence should be addressed.
Abstract
The Yellow Mosaic Disease is the main constraint for decreased yield in blackgram production. Coat protein gene of Mungbean yellow mosaic virus (MYMV) has been characterized and phylogenetic tree was constructed. The genomic DNA from diseased plants was collected and it was tested for it’s quality and quantity using a Nanodrop. The isolated DNA was subjected to polymerase chain reaction using begomovirus universal primers for preliminary confirmation of the virus. The samples amplified for universal primers were subjected for gene specific amplification using DNA A primers. The DNA A primers resulted in the amplification of the expected size of 1000 bp amplicon. The DNA fragments were sequenced and the sequence results of DNA A were compared with other sequences available in NCBI and it showed higher similarity with Mungbean yellow mosaic virus banglore coat protein gene (accession no: MK409376). The sequence was submitted in NCBI database and accession numbers were obtained. The phylogenetic tree was constructed using mega 11 software by comparing the selected strains with our isolate.
Keywords: Blackgram, mosaic, coat protein, characterization, sequence, phylogeny