Screening of NCLB Disease and QTLs Mapping in Zea mays
R. P. Srivastava
Department of Agriculture Chandigarh School of Business Jhanjeri Mohali Punjab, India.
Rajesh Singh
Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University,Varanasi, India.
B. K. Sarma
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.
Saket Kumar *
Department of Environment and Sustainable Development, IESD, BHU, Varanasi-211005, India.
A. Varma
Quantum University Roorkee, Uttrakhand, India.
P. K. Verma
Quantum University Roorkee, Uttrakhand, India.
*Author to whom correspondence should be addressed.
Abstract
Northern Corn Leaf Blight (NCLB) is a foliar disease, caused by a fungal pathogen (Setosphaeria turcica) that damages maize crop due to heavy losses in every year. A study was conducted for NCLB and identified quantitative trait loci (QTLs) in maize. Mapping population F2:3 families was developed involving two inbreds viz CM 212 (susceptible) & CM 145 (resistant).The F2:3 families mapping population was evaluated in two environments (BHU, Varanasi and Nagenahalli, Mandya) for resistance to NCLB. In the polymorphic survey about 360 pairs of simple sequence repeat (SSR) primer used between two parents (CM 212 & CM 145)and identified 54 polymorphic markers. Data was recorded for disease severity traits viz Percent Disease Index (PDI), Area Under Disease Progress Curve (AUDPC) based on PDI, Lesion Area, AUDPC based on lesion area in QTL Mapping. The QTLs were identified interestingly, on 4th chromosome viz: 4.03/4.05, 4.08/4.1, 4/4.07 and 4.08/4.1 from observed disease severity in both environments. The initially two QTLs (QTL-1 & QTL-2) detected with trait Percent Disease Index and other two (QTL 3 & QTL 4) detected with AUDPC based on PDI.
Keywords: Zea mays, Setosphaeria turcica, Lesion area, Northern corn leaf blight, QTLs