Utilising Inter-Random Amplified Polymorphic DNA, Assess Genetic Variability in Indigenous Medicinal Plant Heart-leaved Moonseed (Tinospora cordifolia)
B. C. Akhilraj *
Department of Plantation, Spices, Medicinal and Aromatic Crops, HC & RI, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
J. Suresh
Coconut Research Station, Tamil Nadu Agricultural University, Aliyar Nagar, Pollachi, Tamil Nadu, India.
K. Rajamani
Department of Floriculture and Landscape Architecture, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
M. Kumar
ICAR- Krishi Vigyan Kendra, Tamil Nadu Agricultural University, Tindivanam, Villupuram, Tamil Nadu, India.
R. Gnanam
Department of Bio Informatics, CPMB & B, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
The Ayurvedic medical system, which uses herbal preparations for its traditional remedies, has been used in India since ancient times. One of the powerful immunomodulators is Tinospora cordifolia, also known as AMRITA, which is credited to this miracle medication for giving its user youth, longevity, and vigour. RAPD has been widely and successfully used to identify several species of medicinally significant plant. The need for research into and protection of therapeutic plant species is growing. The perseverance of the evaluation was to assess the genetic diversity among 10 accessions of T. cordifolia obtained from various districts in Tamil Nadu. Eighteen decamer primers were used in the study. Nine of them had repeatable, different polymorphism patterns in their primers. The DNA recovered from several T. cordifolia accessions exhibited random amplification and various levels of genetic polymorphism was observed. In the sample from Salem district the most genetic polymorphism was found.
Keywords: Tinospora cordifolia, Tamil Nadu, RAPD, genetic diversity, medicinal plant