Enhancing Micro-propagation of Some Cameroonian Plantain (Musa paradisiaca L) Cultivars/Landraces

Main Article Content

Dominic, K. Njualem
Tange Denis Achiri
Amanwi Climson Yuninwenkeh
Abdulai Assan Nkuh
Fornkwa Victorine Yaya

Abstract

Aims: This study was conducted to enhance germplasm conservation of some indigenous plantain landraces through tissue culture techniques in Cameroon.

Study Design: The experiment was laid out in a completely randomized design (CRD) with three treatments in four replications.

Place and Duration of Study: The study was conducted in the tissue culture laboratory of the Institute of Agricultural Research for Development (IRAD), Bambui, Cameroon, in the first half of 2018.

Methodology: Explants were gotten from three plantain land races (Kwah, Ngumbe and Sangmoh) gotten from Bambui. Shoot tips were excised and cultured on Murashige and Skoog (MS) medium supplemented with 30 g of sucrose, 5 ml of ascorbic acid, 4 ml of 6- benzylaminopurine (BAP 1 mg/l), 1 ml indole-3- acetic acid (IAA 1 mg/l) and 6 g of agar at pH of 5.8±0.1 for shoot initiation and proliferation. Data was collected over a period of 12 weeks every 4th week on the average number of buds, shoot length, number of leaves, number contaminated and number dead.

Results: All the landraces responded positively to shoot tip culture, since they could adapt and regenerate by producing buds with Ngumbe giving the highest mean number (7.0) of buds and Sangmoh giving the lowest (3.0). Analysis of variance revealed significant differences (p = 0.05) in most of the parameters measured except for number contaminated. High dead rate was recorded in Kwah with dead mean of 3.0. Ngumbe was found to regenerate better than Kwah and Sangmoh.

Conclusion: It is recommended that the three plantain landraces, germplasm be conserved. The ramification of this finding vis-à-vis germplasm conservation and increase productivity is explained.

Keywords:
Plantain, explants, shoot tip culture, germplasm conservation, IRAD Bambui, Musa paradisiaca.

Article Details

How to Cite
Njualem, D. K., Achiri, T. D., Yuninwenkeh, A. C., Nkuh, A., & Yaya, F. V. (2019). Enhancing Micro-propagation of Some Cameroonian Plantain (Musa paradisiaca L) Cultivars/Landraces. International Journal of Plant & Soil Science, 28(6), 1-10. https://doi.org/10.9734/ijpss/2019/v28i630128
Section
Original Research Article

References

Denham TP, Haberle SG, Lentfer C, Fullager R, Field J, Therin M, Porch M, Winsborough M. Origins of agriculture at Kuk swamp in the Highlands of New Guinea. Science. 2003;301:189-193.

Simmonds NW, Shepherd K. The taxonomy and origins of the cultivated bananas. Botanical Journal of the Linnean Society. 1955;55:302-312.

Heslop-Harrison JS, Schwarzacher T. Domestication, genomics and the future for banana. Annal of Botany. 2007;100:1073-1084.

Perrier X, Langhe ED, Donohue M, Lentfer C, Vrydaghs L, et al. Multidisciplinary perspectives on banana (Musa spp.) domestication. Proceeding of National Academy of Science USA. 2011;8:11311-11318.

Scot CN, Ploetz RC, Kepler AK. Musa species (banana and plantain) Musaceae (banana family). Species Profile for Pacific Island Agroforestry. 2006;2(2).
(Accessed 12 June 2018)
Available:www.traditionaltree.org

Nayar NM. The bananas: Botany, origin and dispersal. Horticultural Review. 2010;36:117-164.

Arvanitoyannis IS, Mavromatis G, Gramatikaki-Avgeli, Sakellariou M. Banana: Cultivars, biotechnological approaches and genetic transformation. International Journal of Food Science Technology. 2008;43:1871-1879.

Fogain R, Ysenbrandt H. Utilisation of neem (Azadirachta indica) and mushroom (Beauveria bassiana) against weevil of bananas and plantains. Proceedings of the Vth Annual Conference on Bioscience and Food Security. 1998;223-229.

Anon. Practical guide on cultivation of banana plantain in Cameroon. MINADER/PPTE, October Edition; 2005.
(Accessed 10 January 2019)
Available:www.programme plantain.com

Hauser S, Amougou D. Plantain (Musa spp.) cropping systems of Southern Cameroon. In T. Dubois, S. Hauser, C. Staver, and D. Coyne (Eds.), Proceedings of an International Conference on Banana and Plantain in Africa Harnessing International Partnerships to Increase Research Impact. Acta Horticulturae. 2010;879:495–508.

Temple L, Bikoï A, Chataigner J. La consommation de banane plantain au Cameroun. Les cahiers de la recherche-développement. Systèmes Agroalimentaires à Base de Racines, Tubercules et Plantains. 1997;44:73–85.

Buah JN, Tachie-Menson JW. Suitability of bud manipulation techniques as an alternative to tissue culture in the production of suckers for plantains and bananas. Biotechnology. 2015;14(1):41-46.

Ray T, Dutta I, Saha P, Das D, Roy SC. Genetic stability of three economically important micropropagated banana (Musa sp) cultivars of lower Indo-Gangetic plains, as assessed by RAPD and ISSR markers. Plant Cell Tissue Org. Cult. 2006;85:11-21.

Rowe P, Rosales FE. Bananas and plantains. Fruit Breed. 1996;1:167-211.

Vuylsteke D, Ortiz R, Ferris RSB, Crouch JH. Plantain improvement. Plant Breed. Review. 1997;14:267-320.

Ortiz R, Vuylsteke D. Recent advances in Musa genetics, breeding and biotechno-logy. Plant Breed Abs. 1996;66:1355-1363.

Pushpangadan P, Behl HM. Environment & Biodiversity: Agenda for future. ICPEP. 2005;3.
(Accessed 17 September 2018)
Available:http://www.geocities.com/isebindia/ICPEP-3/ICPEP3-S-2.html

Ndenecho EN. Implications of fuel wood yield, availability and harvest in Tubah Mountain Forest, Cameroon. Global Journal of Human Social Science. 2009;10:49-55.

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 1962;15:473-497.

Sipen P, Davey MR. Effects of N6benzylaminopurine and indoleacetic acid on in vitro shoot multiplication, nodulelike meristem proliferation and plant regeneration of Malaysian bananas. Trop. Life Sci. Res. 2012;23(2):67-80.

Rahman S, Biswas N, Hassan MM, Ahmed MG, Mamun ANK, Islam MR, Moniruzzaman M, Haque ME. Micropropagation of banana (Musa sp.) cv. Agnishwar by in vitro shoot tip culture. Intl. Res. J. Biotech. 2013;4(4):83-88.

Iqbal MM, Muhammad A, Hussain I, Bilal H. Optimization of in vitro micropropagation protocol for banana (Musa sapientum L.) under different hormonal concentrations and growth media. Intl. J Agric. Innov. Res. 2013;2(1):23-27.

Hirimburegama K, Gamage N. Cultivar specificity with respect to in vitro micropropagation of Musa spp. (banana and plaintain). Horti. Sci. 1997;75:205- 211.

Saha-Roy O, Bantawa P, Gosh SK, Texeira da Silva JI, Deb-Ghosh P, Mondal TK. Micropropagation and field performance of ‘Malbhog’ (Musa paradesiaca, AAB group): A popular banana cultivar with high keeping quality of North East India. Tree and Forestry Sci. Biotech. 2010;4:(Special Issue 1):52-58.

Vuylsteke D, De Langhe E. Feasibility of in vitro propagation of bananas and plantains. Tropical Agriculturist. (Trinidad). 1985;62:323-328.

Vuylsteke D, Wilson GF. Genetic stability of in vitro propagated plantains. (Musa cv. AAB). In: Proceediligs of Sixth International Congress of Plant Tissue and Cell Culture (Ed. D.A. Somers). University of Minnesota, USA. 1989;258.

Ferdous MH, Masum BAA, Mehraj H, Taufique T, Jamal-Uddin AFM. BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture. Journal of Bioscience and Agriculture Research. 2015;03(02):87-95.

Rabbani MG, Ali MH, Mondal MF. Effect of BAP and IBA on micropropagation of some banana cultivars. Bangladesh Hort. 1996;25(1&2):47-52.

Enjalric F, Carron MP, Lardet L. Contamination of primary cultures in tropical areas: The case of Hevea braseliensis. In: Bacterial and Bacteria Like Contaminants of Plant Tissue Culture. Ishs Acts Horticulture. 1988;225.
(Accessed 10 March 2019)
Available:Http://www.actahort.org/bools/2225/in-dex.htm

Titov SSK, Bhowmik A, Mandal A, Alam MS, Uddin SN. Control of phenolic compound secretion and effects of growth regulators for organ formation from Musa spp. cv. Kanthali floral bud explants. Am. J. Biochem Biotech. 2006;2:97104.

Ko WH, Su CC, Chen CI, Chao CP. Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formasana with ascorbic acid. Plant Cell Tissue. Organ Culture. 2009;96:137-141.

Ahmad I, Hussain T, Ashraf I, Nafees M, Maryam MR, Iqbal M. Lethal effect of secondary metabolites on plant tissue culture. American-Eurasian J. Agric and Environ. Sci. 2013;13(4):539-547.

Palmer JK. Banana polyphenol oxidase preparation and properties. Plant Physiology. 1963;38:508-513.