Influence of Zinc Oxide Nanoparticles on Soil Physicochemical Properties and Arachis hypogea Rhizosphere Microbial Community

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Progress Oghenerume
Samuel Eduok
Basil Ita
Ofonime John
Inemesit Bassey


We evaluated the effect of 4000 mg zinc oxide (ZnO, 99%, 30 nm) nanoparticle on the physicochemical and microbiological properties of organic manure amended ultisol and loam soil cultivated with Arachis hypogaea using standard methods. The results indicate varying effects on the physicochemical properties in relation to the soil type. The pH of the control ultisol at 7.85 ± 0.17 and 8.3 ± 0.12 in the amended ultisol whereas, the control loam was 7.15 ± 0.17 and 7.41 ± 0.11 in the amended soil indicating 1.06- and 1.04-times higher difference than the controls respectively.  Phosphorus concentration at 57.82 ± 0.54%, 50.81 ± 0.22% and 55.97 ± 0.04%, 59.97 ± 0.02% was 1.14 times lower in the ZnO amended ultisol and 1.07 times higher in amended loam soil compared to the respective controls. The organic matter content in the control and amended ultisol was 2.28 ± 0.32% and 0.91 ± 0.02%, 3.68 ± 0.36% and 0.36 ± 0.02% in the control and amended loam soil. The concentration of nitrate in the control ultisol was 0.05 ± 0.01% and 0.03 ± 0.01% in the amended soil. The nitrate in the control loam soil was 0.08 ± 0.01% relative to 0.02 ± 0.01% in the treated soil and these differences were significant at p = 0.05. The concentration of nutritive salts was reduced and in contrast iron, copper, exchangeable acids, exchange capacity, clay and silt increased in the amended soils. Further to this, heterotrophic ammonia and nitrate-oxidizing bacterial population were inhibited in the amended soils and denitrifying organisms were stimulated. The organisms were members of the genera Pseudomonas, Xanthobacter, Enterobacter, Bacillus, Lactobacillus, Citrobacter, Nitrosomonas, Agromyces and Rhizobium. ZnO nanoparticles altered the soil physicochemical properties which exacerbated the negative effect on microbial abundance and varied with the soil type.

Ultisol, loam soil, ZnO nanoparticle, bacterial abundance, soil properties.

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How to Cite
Oghenerume, P., Eduok, S., Ita, B., John, O., & Bassey, I. (2020). Influence of Zinc Oxide Nanoparticles on Soil Physicochemical Properties and Arachis hypogea Rhizosphere Microbial Community. International Journal of Plant & Soil Science, 32(8), 88-100.
Original Research Article


Dimesh RA, Manonmani S, Hamza V. Engineered nanoparticles in the soil and their potential implications to microbial activity. Geoderma, 2012;19(27):173-174.

Zhang Y, Yu-Rui L, Robert JA, Michael R. Inventory of engineered nanoparticle-containing consumer products available in the singapore retail market and likelihood of release into the aquatic environment. Inter J Environ Res Public Health. 2015;12:8717–8743.

Carbone C, Murugaboopathib G, Johna A, Sivakumarc R, Ganes R, Krithikae S, Priyae G. Significance of nanotechnology in food industry Apcbee Procedia. 2014;8:109–113.

Eduok S, Hendry C, Ferguson R, Martin B, Villa R, Jefferson B, Coulon F. Insights into the effect of mixed engineered nanoparticles on activated sludge performance. Fems Microbiol Ecol. 2015;91(7):Fiv082

Batley GE, Kirby JK, McLaughlin MJ. Fate and risks of nanomaterials in aquatic and terrestrial environments. Acc Chem Res. 2012;46:854-864.

Javed Z, Dashora K, Mishra M, Fasake VD, Srivastva A. Effect of accumulation of nanoparticles in soil health- a concern on future. Front Nanosci Nanotechnol. 2019;5.

DOI: 10.15761/FNN.1000182.

Alves ML, Oliveira F, Luís CI, Nogueira PO, André JF, Márcio AB, Baretta CR. Influence of ZnO nanoparticles and a non-nano ZnO on survival and reproduction of earthworm and springtail in tropical natural soil. Revista Brasileira de Ciência do Solo. 2019;43:e0180133

Shi J, Jien Y, Huaxiang F, Shu Z, Chen X. Effects of copper oxide nanoparticles on paddy soil properties and components. Nanomater. 2018;8:839

Romero-Freire A, Lofts S, Martín-Peinado FJ, van Gestel CA. Effects of aging and soil properties on zinc oxide nanoparticle availability and its ecotoxicological effects to the earthworm Eisenia andrei. Environ Toxicol Chem. 2017;36:137-46.

Cullen LG, Tilston EL, Mitchell GR, Collins CD, Shaw LJ. Assessing the impact of nano-and micro-scale zerovalent iron particles on soil microbial activities: Particle reactivity interferes with assay conditions and interpretation of genuine microbial effects. Chemos. 2011;82:1675–1682.

Hazelton PA, Murphy BW. Interpreting soil test results: What do all the numbers mean? CSIRO Publishing: Melbourne; 2007.

Sorensen PC, Oelsligle DD, Trudser D. Extraction of Zn, Fe and Mn from 1971 soils with 0.1 N hydrochloric acid as affected by soil properties, solution: soil ratio, and length of extraction period. Soil Sci. 1971;111:352-159.

Liu JY, Wang ZY, Liu FD, Kane AB, Hurt RH. Chemical transformations of nanosilver in biological environments. ACS Nanotechnol. 2012;6:9887–9899.

Elliot DW, Zhang WX. Field Assessment of nanoscale bimetallic particles for groundwater treatment Environ Sci Technol. 2001;35:4922-4926.

Ben-Moshe T, Frenk S, Dror I, Minz D, Berkowitz B. Effects of metal oxide nanoparticles on soil properties. Chemos. 2013;90:640-646.

Oghenerume P, Eduok S, Ita B, John O, Bassey I. Impact of zinc oxide nanoparticles amended organic manure on Arachis hypogaea growth response and rhizosphere bacterial community. Inter J Plant Soil Sci. 2020;32(5):24-35.

Association of official analytical chemists (AOAC). Official methods of analysis (19th Edition) AOAC. Washington DC; 2012.

Udo EJ, Ibia TO, Ogunwale JA, Ano AO, Esu IE. Manual of soil, plant and water analysis. Sibon Books Limited, Lagos; 2009

Cheesbrough M. District laboratory practice in tropical countries. Cambridge University Press; 2006

Holt JG, Krieg NR, Sneath PHA, Stanley JT, William ST. Bargey’s manual of determinative bacteriology. Williams and Wilikins, Baltimore; 1994.

Voegelin A, Pfister S, Scheinost AC, Marcus MA, Kretzschmar R. Changes in zinc speciation in field soil after contamination with zinc oxide. Environ Sci Technol. 2005;39:6616–6623.

Ashraf M. Organic substances responsible for salt tolerance in Eruca sativa. Biologia Plantarum. 1994;36:255-259.

Kalteh M, Alipour ZT, Ashraf S, Aliabadi MM, Nosratabadi AF. Effect of silica nanoparticles on basil (Ocimum basilicum) under salinity stress. J Chem Health Risks. 2014;4:49-55.

Camejo D, Guzmán-Cedeño Á, Moreno A. Reactive oxygen species, essential molecules, during plant–pathogen interactions. Plant Physiol Biochem. 2016;103:10-23.

Walker GM. The roles of magnesium in biotechnology. Critical Reviews in Biotechnology. 1994;14(4):311-354.

Hong FH, Zhou J, Liu C, Yang F, Wu C, Zheng L, Yang P. Effect of nano TiO2 on photochemical reaction of chloroplasts of spinach. Biol Trace Element Res. 2005;105(1-3):269-279.

Huber D, Jones J. The role of magnesium in plant disease. Plant Soil. 2013;368(1/2):73-85.

Lee D, Laporta L, Maja BF, Chae M, Zhouxin S, Steven PB, Jordi GO, Gürol MS. Magnesium flux modulates ribosomes to increase bacterial survival. Cell. 2019;177(2):352-360.

Wallace A, Mueller RT. Calcium uptake and distribution in plants. Journal of Plant Nutrition. 1980;2:1-2.

Schulte EE, Kelling KA. Soil and applied copper. Understanding Plant Nutrients, A2527; 2004.

Fitriatin BN, Fatimah I, Sofyan ET. The effect of phosphate solubilizing bacteria and organic fertilizer on phosphatase, available P, P uptake and growth sweet corn in Andisols. Earth Environ Sci. 2018;215:012002.

Rotthauwe JH, Witzel KP, Liesack W. The ammonia monooxygenase structural gene amoA as a functional marker: Molecular fine-scale analysis of natural ammonia-oxidizing populations. Appl Environ Microbiol. 1997;63:4704–4712.

Castellano MJ, Mueller KE, Olk DC, Sawyer JE, Six J. Integrating plant litter quality, soil organic matter stabilization, and the carbon saturation concept. Global Change Biol. 2015;21(105):3200–3209.

Neumann D, Heuer A, Hemkemeyer M, Martens R, Christoph C. Importance of soil organic matter for the diversity of microorganisms involved in the degradation of organic pollutants The ISME J. 2014;8:1289–1300.

Lee HV, Juan JC. Nanocatalysis for the conversion of non-edible biomass to biogasoline via deoxygenation reaction. In: Rai, M., da Silva, S.S. (Eds.), Nanotechnology for Bioenergy and Biofuel Production; 2017.

Available: , 2017

Zhang L, Yuan Z, Jiachao Z, Guangming, Z, Haoran D, Weicheng C, Wei F, Yujun C, Yaoyao W, Qin N. Impacts of iron oxide nanoparticles on organic matter degradation and microbial enzyme activities during agricultural waste composting. Waste Manage. 2019;95:289–297.

Simonin M, Colman B, Anderson MS, Benhardt ES. Engineered nanoparticles interact with nutrients to intensify eutrophication in a wetland ecosystem experiment. Ecol Appl. 2018;19:12.

Ji B, Yang K, Lei Z, Yu J, Hongyu W, Jun Z, Huining Z. Aerobic denitrification: A review of important advances of the last 30 years. Biotechnol Bioproc Eng. 2015;20: 643-651.

Babalola OO, Glick BR. The use of microbial inoculants in African agriculture: current practice and future prospects. J Food Agric Environ. 2012;10:540–549.

Srinivasan R, Mahesh S, Yandigeri B, Sudhanshu K, Ajjanna R, Alagawadi A. (2012). Effect of salt on survival and P-solubilization potential of phosphate solubilizing microorganisms from salt affected soils. Saudi J Biol Sci. 2012;19:427–434.

Lee J, Yong GK, Moo HC, Jintae L. ZnO nanoparticles inhibit Pseudomonas aeruginosa biofilm formation and virulence factor production. Microbiol Res. 2014;169:888–896.

Simonin M, Guyonnet JP, Martins JMF, Ginot M, Richaume A. Influence of soil properties on the toxicity of TiO2 nanoparticles on carbon mineralization and bacterial abundance. J Hazard Mater. 2015;283:529–535.

Cornelis G, Hund-Rinke K, Kuhlbusch T. Fate and bioavailability of engineered nanoparticles in soils: a review. Crit Rev Environ Sci Technol. 2014;44(24):2720-2764.

Meliani A, Bensoltane A, Mederbel K. Microbial diversity and abundance in soil: related to plant and soil type. Amer J Plant Nutri Fert Technol. 2012;2:10-18.

Chavan S, Nadanathangam V. Effects of nanoparticles on plant growth-promoting bacteria in indian agricultural soil Agron. 2019;9(3):140.

Xia T, Kovochich M, Liong M, Madler L, Gilbert B, Shi H, Yeh JI, Zink JI, Nel AE. Comparision of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS Nano. 2008;2:2121-2134.

Rajput V, Minkina T, Sushkova S, Tsitsuashvili V, Mandzhieva S, Gorovtsov A, Nevidomskyaya D, Gromakova N. Effect of nanoparticles on crops and soil microbial communities. J Soils Sediments. 2017;2179–2187.