Genetic and Biochemical Studies of Thymelaea hirsuta L. Growing Naturally at the North Western Coast of Egypt

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Mohamed M. Abd El- Maboud
Mohamed Abd S. El- Zayat


This study aims to elucidate the biochemical and genetic behaviors of Thymelaea hirsuta L. growing naturally at the following habitats; ooletic sand dunes, slope of salt marshes, non-saline depression, road side, plateau, upstream of Wadi Halazeen, and wadi bed of Wadi Halazeen along the western Mediterranean coast of Egypt. Soil and plant samples were collected in May, 2017. Water content recorded the highest value in Thymelaea growing at slope of salt marshes. Total soluble carbohydrate attained the highest value in Thymelaea growing at up-stream of Wadi Halazeen while the highest values in none-souble carbohydrate and proline were observed in plants growing at ooletic sand dunes. Total carbohydrate recorded the highest value in Thymelaea growing at plateaue. Total lipids and total phenols attained the highest content in Thymelaea growing at wadi bed. Genetically, ISSR & SRAP molecular markers were done. Seven ISSR and four SRAP primes produced 73.78% and 68.57% of polymorphism, respectively. PIC value, assay efficiency index, effective multiples ratio and marker index, were higher in SRAP than in ISSR.

ISSR, proline, SRAP, Thymelaea hirsuta.

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How to Cite
Maboud, M. M. A. E.-, & Zayat, M. A. S. E.-. (2020). Genetic and Biochemical Studies of Thymelaea hirsuta L. Growing Naturally at the North Western Coast of Egypt. International Journal of Plant & Soil Science, 32(14), 28-37.
Original Research Article


Anjum SA, Ashraf U, Tanveer M, Khan I, Hussain S, Shahzad B, Zohaib A, Abbas F, Saleem MF, Ali I, Wang LC. Drought induced changes in growth, osmolyte accumulation and antioxidant metabolism of three maize hybrids. Front. Plant Sci. 2017;8:69. DOI: 10.3389/fpls.2017.00069.

Abd El-Maboud MM, Abd Elmonem AA, Ibrahim MF. Biochemical responses of wolfbane (Periploca angustifolia Labill.) to water stress. Int J Plant Soil Sci. 2018;24:1-9. DOI: 10.9734/IJPSS/2018/44540.

Delauney A, Verma D. Proline biosynthesis and osmoregulation in plants. The Plant Journal. 1993;4:215-223.

Šircelj H, Tausz M, Grill D, Batič F. Biochemical responses in leaves of two apple tree cultivars subjected to progressing drought. J. Plant Physiology. 2005;162:1308-1318.

Abd El-Maboud MM, Khalil RM. Ecophysiological and genetic studies on some species of the genus Suaeda Forssk ex Scop. in the Mediterranean Sea coast. World Applied Sciences Journal. 2013;27:811-825. DOI:10.5829/idosi. 27.07.13669.

Li G, Quiros CF. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet. 2001;103:455-461.

Wolfe AD, Liston A. Contributions of PCR-based methods to plant systematics and evolutionary biology. In: Soltis, D.E., Soltis, PS, Doyle JJ. (Eds.), Molecular Systematics of Plants II. Kluwer, Boston, MA. 1998;43-86.

Reddy MP, Sarla N, Siddiq EA. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica. 2002;188:9-17.

Feng F, Chen M, Zhang D, Sui X, Han S. Application of SRAP in the genetic diversity of Pinus koraiensis of different provenances. African Journal of Biotechnology. 2009;8:1000-1008.

Robarts DWH, Wolfe AD. Sequence-related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology. Applications in Plant Sciences. 2014;2. DOI:10.3732/apps.1400017.

Boulos L. Flora of Egypt (V. 2), Cairo: Al Hadara Publishing; 2000.

Kawano M, Matsuyama K, Miyamae Y, Shinmoto H, Elyes Kchouk M, Morio T, Shigemori H, Isoda H. Antimelanogenesis effect of Tunisian herb Thymelaea hirsuta extract on B16 murine melanoma cells. Exp. Dermatol. 2007;16:977-984.

Akrout A, Gonzalez LA, EL Jani H, Madrid PC. Antioxidant and antitumor activities of Artemisia campestris and Thymelaea hersuta from southern Tunisia. Food and Chemical Toxicology. 2011;49:342-347.

Amari NO, Bouzouina M, Berkani A, Lotmani B. Phytochemical screening and antioxidant capacity of the aerial parts of Thymelaea hirsuta L. Asian Pac J Trop Dis. 2014;4:104-109.

El Amrani F, Rhallab A, Alaoui T, El Badaoui K, Chakir S. Hypoglycaemiceffect of T. hirsuta in normal and streptozotocin-induced diabetic rats. J. Med. Plants Res. 2009;3:625-629.

Helal NM, Alharby HF, Alharbi BM, Bamagoos AA, Hashim AM. Thymelaea hirsuta and Echinops spinosus: xerophytic plants with high potential for first generation biodiesel production. Sustainability. 2020;12:1137. DOI: 10.3390.

Doyle JJ, Doyle JL. Isolation of plant DNA from fresh tissue. Focus. 1990;12:13-15.

Kilmer NJ, Alexander LT. Methods of making mechanical analysis of soils. Soil Science. 1949;68:15-24.

Rowell DL. Soil Science: Methods and Applications. Dept of Soil Science, Univ. of Reading. Co-published in the US with John Willey and Sons Inc.; New York; 1994.

Chaplin MF, Kennedy JF. Carbohydrate analysis: A practical approach. 2nd Ed. Oxford Univ., Press Oxford, New York, Tokyo; 1994.

Bates L, Waldren R, Teare, I. Rapid determination of free proline for water-stress studies. Plant and Soil.1973;39:205-207.

Shahidi F, Naczk M. Food phenolics: sources, chemistry, effects, applications. Lancaster: Technomic Publishing Company Inc; 1995.

Casella G. Statistical Design (1st ed.). Gainesville: Springer; 2008.

Michigan State University MSTATC, A software program for design, management and analysis of Agronomic Research Experiments. Michigan State University, East Lansing, MI; 1991.

Yan WP, Li JL, Wu SM, Qin SC, Yang XF, Wang Y. Optimization Techniques for SRAP-PCR Reaction System in Mallotus oblongifolius (Miq.) Muell. Arg. Molecular Plant Breeding. 2018;16(10):3252-3258.

Rohlf FJ. NTSYS-pc, Numerical taxonomy and multivariate analysis system. Version 2.11. New York, Exeter, Setauket; 2000.

Dice LR. Measures of the amount of ecologic association between species. Ecology. 1945;26:297-302.

Mantel N. The detection of disease clustering and a generalized regression approach. Cancer Research. 1967;27:209-220.

Smith JSC, Chin ECL, Shu H, Smith OS, Wall SJ, Senior ML, Mitchel SE, Kresorich S, Tiegle J. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree. Theoretical and Applied Genetics. 1997;95:163-173.

Abd El-Maboud MM, Abd Elbar OH. Adaptive responses of Limoniastrum monopetalum (L.) Boiss. growing naturally at different habitats. Plant Physiol. Rep. 2020;25:325-334.

Du Y, Zhao Q, Chen L, Yao X, Zhang W, Zhang B, Xie F. Effect of drought stress on sugar metabolism in leaves and roots of soybean seedlings. Plant Physiology and Biochemistry. 2020;146:1-12.

Fu J, Huang B, Fry J. Osmotic potential, sucrose level, and activity of sucrose metabolic enzymes in tall fescue in response to deficit irrigation. J Am Soc Hortic Sci. 2010;135:506-510.

Ehsen S, Abideen Z, Rizvi RF, Gulzar S, Aziz I, Gul B, Khan MA, Ansari R. Ecophysiological adaptations and anti-nutritive status of sustainable cattle feed Haloxylon stocksii under saline conditions. Flora. 2019;257. Available:

Peng J, Liu J, Zhang L, Luo J, Dong H, Ma Y, Zhao X, Chen B, Sui N, Zhou Z, Meng Y. Effect of soil salinity on sucrose metabolism in cotton leaves. PLoS One. 2016;11(5):e0156241.

Abd El-Maboud MM. Mechanisms of drought tolerance in Cornulaca monacantha Del. Journal of Environmental Sciences. 2016;45:175-186.

Schmid KM, Ohlrogge JB. Lipid metabolism in plants. In: Biochemistry of lipids, lipoproteins and membranes (4th edn.) D.E. Vance and J.E. Vance (Eds.); 2002.

Kim H. Lipid metabolism in plants. Plants. 2020;9. DOI:10.3390/plants9070871.

Szabados L, Savouré A. Proline: A multifunctional amino acid. Trends in Plant Science. 2010;15:89-97. DOI:

Li S, Zhang N, Zho X, Ma R, Yang J, Tang X, Si H. Enhanced drought tolerance with artificial microRNA-mediated StProDH1 gene silencing in potato. Crop Science; 2020. DOI: 10.1002/csc2.20064.

Shui G, Leong LP. Separation and determination of organic acids and phenolic compounds in fruit juices and drinks by high-performance liquid chromatography. Journal of Chromatography A. 2002;977:89-96.

Abd El-Maboud MM. Seasonal variations effect on antioxidant compounds and their role in the adaptation of some halophytes at Wadi Gharandal, Southwest Sinai. Annals of Agricultural Science; 2019. DOI:10.1016/j.aoas.2019.11.001

Yao H, Zhao Y, Chen DF, Chen JK, Zhou TS. ISSR primer screening and preliminary evaluation of genetic diversity in wild populations of Glycyrrhiza uralensis. Biologia Plantarum. 2008;52:117-120.

Verma KS, Haq S, Kachhwaha S. RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress. 3 Biotech. 2017;7:288. DOI 10.1007/s13205-017-0918-z.

Cichorz S, Gośka M, Litwiniec A. Miscanthus: Genetic diversity and genotype identification using ISSR and RAPD markers. Molecular Biotechnology. 2014;56:911-924. DOI:10.1007/s12033-014-9770-0.

Aneja B, Yadav NR, Yadav RC, Kumar R. Sequence related amplified polymorphism (SRAP) analysis for genetic diversity and micronutrient content among gene pools in mungbean [Vigna radiata (L.) Wilczek]. Physiol Mol Biol Plants. 2013;19(3):399-407.

Alawhibi MS, Alsubeie MS, Bukhari NA, Soliman DA. Molecular characterization of Haloxylon salicornicum Moq. In Saudi Arabia. Saudi Journal of Biological Sciences. 2020;27:1824-1829.