In vitro Evaluation of Antifungal Activity of Different Trichoderma spp. and Plant Extracts against Sclerotinia sclerotiorum (Lib.) de Bary Causing Stem Rot of Mustard
Md Zulkar Nain
Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
R. U. Khan
Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
Vaibhav Pratap Singh *
Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
Sibte Sayyeda
Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
*Author to whom correspondence should be addressed.
Abstract
Sclerotinia stem rot (SSR) is one of the most destructive disease of mustard which affects the quality and quantity of the crop and resulting in severe yield losses. The present study was conducted to evaluate the bio-efficacy of different Trichoderma spp. and plant extracts against Sclerotinia sclerotiorum under laboratory conditions. All the tested Trichoderma spp. and plant extracts showed a significant effect on growth inhibition of test fungus over control. Among different Trichoderma spp., maximum growth inhibition (73.33%) was observed with T. viride followed by T. harzianum (67.77%). While as the least efficacy was found in T. atroviride (38.37%) against the pathogen. Of all, Allium sativum was proved to be most potent at all three tested concentrations (5, 10, and 15%), thereby registering 59.26, 67.41, and 100.0% growth inhibition followed by Azadirachta indica and Lantana camara, respectively. However, Argemone maxicana was least effective at all concentrations in this study.
Keywords: Trichoderma spp, plant extracts, rapeseed-mustard, sclerotinia stem rot, in vitro