In vitro Evaluation of Antifungal Activity of Different Trichoderma spp. and Plant Extracts against Sclerotinia sclerotiorum (Lib.) de Bary Causing Stem Rot of Mustard
Md Zulkar Nain
Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
R. U. Khan
Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
Vaibhav Pratap Singh *
Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
Sibte Sayyeda
Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
*Author to whom correspondence should be addressed.
Abstract
Sclerotinia stem rot (SSR) is one of the most destructive disease of mustard which affects the quality and quantity of the crop and resulting in severe yield losses. The present study was conducted to evaluate the bio-efficacy of different Trichoderma spp. and plant extracts against Sclerotinia sclerotiorum under laboratory conditions. All the tested Trichoderma spp. and plant extracts showed a significant effect on growth inhibition of test fungus over control. Among different Trichoderma spp., maximum growth inhibition (73.33%) was observed with T. viride followed by T. harzianum (67.77%). While as the least efficacy was found in T. atroviride (38.37%) against the pathogen. Of all, Allium sativum was proved to be most potent at all three tested concentrations (5, 10, and 15%), thereby registering 59.26, 67.41, and 100.0% growth inhibition followed by Azadirachta indica and Lantana camara, respectively. However, Argemone maxicana was least effective at all concentrations in this study.
Keywords: Trichoderma spp, plant extracts, rapeseed-mustard, sclerotinia stem rot, in vitro
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Yadav MS, Godika S, Yadava DK, Ahmad N, Mehta N, Bhatnagar K, Agrawal VK, Kumar A, Thomas L, Chattopadhyay C. Prioritizing components of package of integrated pest management in Indian mustard (Brassica juncea) in India for better economic benefit. Crop Protection. 2019;120:21-29.
Chand S, Prakash Patidar O, Chaudhary R, Saroj R, Chandra K, Kamal Meena V, Vasisth P. Rapeseed-mustard breeding in India: Scenario, achievements and research needs. Intech Open; 2021. DOI: 10.5772/intechopen.96319
Agricultural Statistics at a Glance. Directorate of Economics and Statistics Department of Agriculture and Farmers Welfare Ministry of Agriculture and Farmers Welfare Government of India; 2021.
Saharan GS. Management of rapeseed and mustard diseases. In: Advances in oilseed research. Rapeseed and Mustard (Kumar D. and Rai M, eds.) Scientific Pub. Jodhpur. 1992;1:155-188.
Saha LR, Singh HB, Raychaudhuri SP, Verma JP. Diseases of rapeseed and mustard and their management. Rev. Tropical Pl. Pathol. 1989;5:47-77.
Purdy LH. Sclerotinia sclerotiorum: History, diseases and symptomatology, host range, geographic distribution, and impact. Phytopathology. 1979;69:875-880.
Saharan GS, Mehta N. Sclerotinia diseases of crop plants: Biology, ecology and disease management: Springer Press. Sclerotinia sclerotiorum Isolates in Northern Parts of Iran. World Appl. Sci. J, 2008;8(3):326-333.
Sharma P, Meena PD, Singh D. Effect of Sclerotinia sclerotiorum culture filtrate on seed germination and seedling vigour of Indian mustard (Brassica juncea cv. Rohini). J Oilseed Brassica. 2014;5:158-161.
Kolte SJ. Diseases of annual edible oilseed crops, rapeseed-mustard and sesame diseases. CRC Press Inc Boca Raton. 1985;2:135.
Parveen K, Haseeb A, Shukla PK. Management of Sclerotinia sclerotiorum on Mentha arvensis C.V.Gomti. J. Mycol. Pl. Pathol. 2007;37(1):33-36.
Saharan GS, Mehta N. Fungal diseases of rapeseed-mustard. In: Diseases of Field Crops (Gupta, VK. and Paul YS. eds.). Indus Publishing Company, New Delhi. 2002:193-228.
Ghasolia RP, Shivpuri A, Bhargava AK. Sclerotinia rot of Indian mustard (Brassica juncea) in Rajasthan. Indian Phytopathol. 2004;57:76-79.
Shukla AK. Estimation of yield losses to Indian mustard (Brassica juncea) due to sclerotinia stem rot. J Phytol Res. 2005;18:267-268.
Bolton DM, Thomma PHJB, Nelson DB. Sclerotinia sclerotiorum (Lib.) de Bary: Biology and molecular traits of a cosmopolitan pathogen. Mol Plant Pathol. 2006;7:1-16.
Wu BM, Subbarao KV. Effects of soil temperature, moisture and burial depths on carpogenic germination of Sclerotinia sclerotiorum and S. minor. Phytopathology. 2008;98:1144–1152.
Mondal B, Khatua DC, Hansda S, Sharma R. Addition to the host range of Sclerotinia sclerotiorum in West Bengal. Scholars Academic Journal of Biosciences. 2015;3:361-364.
Smolinska U, Kowalska B. Biological control of the soil-borne fungal pathogen Sclerotinia sclerotiorum a review. Journal of Plant Pathology. 2018;100:1-12.
Vincent JM. Distortion of fungal hyphae in the presence of certain inhibitors. Nature. 1947;159:239-241.
Dubey SC, Patel B. Evaluation of fungal antagonist against Thanatephorus cucumeris causing web blight urd and mung bean. Indian Phytopath. 2001;54:206–209.
Pandey P, Kumar R, Mishra P. Integrated approach for the management of Sclerotinia sclerotiorum (Lib.) de Bary, causing stem rot of chickpea. Indian Phytopath. 2011;64(1):37-40.
Yadav M, Rakholiya KB, Pawar DM. Evaluation of bioagents for management of the onion purple blotch and bulb yield loss assessment under field conditions. The Bioscan. 2011;8(4):1295-1298.
Sharma P, Meena PD, Singh S, Rai PK. Efficacy of micro-nutrients, fungicides and bio-agents against sclerotinia stem rot (Sclerotinia sclerotiorum) of Indian mustard. Int. J. Curr. Microbiol. App. Sci. 2017;6(10):620-626.
Mehta N, Hieu N, Sangwan M. Efficacy of various antagonistic isolates and species of against causing white stem rot of mustard. Journal of Mycology and Plant Pathology. 2012;42(2):244-250.
Shivpuri A, Gupta RBL. Evaluation of different fungicides and plant extracts against Sclerotinia sclerotiorum causing stem rot of mustard. Phytopathology. 2001;52:272-274.
Vinale F, Sivasithamparam K, Ghisalberti EL, Marra R, Woo SL, Lorito M. Trichoderma–plant–pathogen interactions. Soil Biology and Biochemistry. 2008;40(1):1-10.
Geraldine A, Fabyano L, Carvalho C, Diego D, Elder B, Amanda R, Ulhoa C, Murillo LJ. Cell wall-degrading enzymes and parasitism of sclerotia are key factors on field biocontrol of white mold by Trichoderma spp. Biological Control. 2013;67:308-316.
López-Mondéjar R, Ros M, Pascual J. Added-value of Trichoderma amended compost as biopesticide organic substrates: Alternative to traditional organic substrates. Acta Horticulturae. 2011;898:189-196.
Kapil R, Kapoor AS. Management of white rot of pea incited by Sclerotinia sclerotiorum using Trichoderma spp. and biopesticides. Indian Phytopathology. 2005;58:10-16.
Tripathi AK, Tripathi SC. Management of sclerotinia stem rot of Indian mustard through plant extracts. Vegetos. 2009;22(1):1-3.
Mehta N, Hieu NT, Sangwan MS. Efficacy of some botanicals against Sclerotinia sclerotiorum inciting white stem rot of rapeseed-mustard. Plant Dis Res. 2011;26:82-86.
Meena PD, Gour RB, Gupta JC, Singh HK, Awasthi RP, Netam RS, Godika S, Sandhu PS, Prasad R, Rathi AS, Rai D, Thomas L, Patel GA, Chattopadhyay C. Non-chemical agents provide tenable, eco-friendly alternatives for the management of the major diseases devastating Indian mustard (Brassica juncea) in India. Crop Protection. 2013; 53:169-174.
Fatehpuria PK, Sasode RS, Pandya RK, Singh R, Gupta JC. Efficacy of different inoculation techniques for testing the pathogenicity of Sclerotinia sclerotiorum causing sclerotinia blight of Brassica juncea. IJCS. 2017;5(5):1937-1940.
Fagodia RK, Godika S, Fagodia BL. Effect of physical parameters on the growth and sclerotia formation of Sclerotinia sclerotiorum (Lib.) de Bary, causing stem rot of coriander. Annals of Plant and Soil Research. 2017;19(1):54– 58.
Dennis C, Webster J. Antagonistic properties of species groups of Trichoderma - III. Production of volatile antibiotics. Trans. Br. Mycol. Soc. 1971;51:363-369.