Effect of Nitrogen-fixing Bacteria on Germination, Seedling Vigour and Growth of Two Rice (Oryza sativa L.) Cultivars
International Journal of Plant & Soil Science,
Page 94-106
DOI:
10.9734/ijpss/2022/v34i1631028
Abstract
To evaluate the effect of isolated nitrogen fixing plant growth-promoting bacteria (PGPB) on seed germination and growth promotion of rice cultivars (cv. BPT 5204 and Improved Samba Mahsuri). Eight promising N-fixing PGPB along with two standard cultures (viz. B. japonicum and G. diazotrophicus) were inoculated as seed treatment to rice genotypes and the effect on seed germination, seed vigour index and plant growth promotion of rice cultivars was assessed under in vitro (agar method) and in vivo (pot experiment) net house conditions. PGPBs (viz., Paenibacillus sonchi IIRRBNF1, Paenibacillus sp. IIRRNF2, Ochrobactrum sp. IIRRNF3, Burkholderia cepacia IIRRNF4, Burkholderia sp. IIRRNF5, Stenotrophomonas sp. IIRRNF6, Rhizobium sp. IIRRNF7 and Xanthomonas sacchari IIRRNF8) were enhanced seed germination, seed vigour index, seedling growth and dry matter accumulation (root and shoot dry matter) of rice cultivars under in vitro as well as in vivo conditions. Among all PGPB, Paenibacillus sonchi IIRRBNF1 exhibited the highest ability to stimulate plant growth promotion under both the conditions. The eight PGPB isolates exhibited positive influence on seed germination indices as well as growth promotion traits of rice cultivars at seedling stage and can be further evaluated at different growth stages under pot and field experiment.
Keywords:
- Plant growth promoting bacteria
- nitrogen fixation
- rice seed germination
- rice seedling growth
How to Cite
Bandeppa, S., Phule, A. S., Rajani, G., Babu, K. V. P., Barbadikar, K. M., Babu, M. B. B. P., Mandal, P. K., Sundaram, R. M., & Latha, P. C. (2022). Effect of Nitrogen-fixing Bacteria on Germination, Seedling Vigour and Growth of Two Rice (Oryza sativa L.) Cultivars. International Journal of Plant & Soil Science, 34(16), 94-106. https://doi.org/10.9734/ijpss/2022/v34i1631028
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Zahir ZA, Arshad A, Frankenberger WT. Plant growth promoting rhizobacteria: application and prospectives in agriculture. Adv. Agron. 2004;81:97-168.
Muthukumarasamy R, Cleenwerck I, Revathi G, Vadivelu M, Janssens D, Hoste B, Gum KU, Ki-Do P, Son CY, Sa T, Caballero-Mellado J. Natural association of Gluconacetobacter diazotrophicus and diazotrophic Acetobacter peroxydans with wetland rice. Syst. Appl. Microbiol. 2005; 28(3):277-286.
Govindarajan M, Balandreau J, Kwon SW, Weon HY, Lakshminarasimhan C. Effects of inoculation of Burkholderia vietnamensis and related endophytic diazotrophic bacteria on grain yield of rice. Microbiol. Ecol. 2008;55:2-37.
Silva R, Filgueiras L, Santos B, Coelho M, Silva M, Estrada-Bonilla G, Vidal M, Baldani JI, Meneses C. Gluconacetobacter diazotrophicus changes the molecular mechanisms of root development in Oryza sativa L. growing under water stress. Int. J. Mol. Sci. 2020;21:333.
Schultz N, Pereira W, de Albuquerque Silva P, Baldani JI, Boddey RM, Alves BJR, Urquiaga S, Reis VM. Yield of sugarcane varieties and their sugar quality grown in different soil types and inoculated with a diazotrophic bacteria consortium. Plant Production Science. 2017;1-9.
Dal Cortivo C, Barion G, Visioli G, Mattarozzi M, Mosca G, Vamerali T. Increased root growth and nitrogen accumulation in common wheat following PGPR inoculation: assessment of plant-microbe interactions by ESEM. Agric. Ecosyst. Environ. 2017;247:396-408.
De Souza R, Ambrosini A, Passaglia LMP. Plant growth-promoting bacteria as inoculants in agricultural soils. Genet. Mol. Biol. 2015;38:401-419.
Ladha JK, Reddy PM. Nitrogen fixation in rice systems: State of knowledge and future prospects Plant Soil. 2003;252:151-167.
Araujo AES, Baldani VLD, Galisa PS, Pereira JA, Baldani JI. Response of traditional upland rice varieties to inoculation with selected diazotrophic bacteria isolated from rice cropped at the Northeast region of Brazil. App. Soil Ecol. 2013;64:49-55.
Rogers C, Oldroyd GED. Synthetic biology approaches to engineering the nitrogen symbiosis in cereals. J. Exp. Bot. 2014;65: 1939-1946.
Singh RK, Singh P, Li HB, Yang LT, Li YR. Soil-plant-microbe interactions” use of nitrogen-fixing bacteria for plant growth and development in sugarcane. In: Singh DP, et al. editors. “Plant-Microbe Interac-tions in Agro-Ecological Perspectives”. Singapore Pte Ltd. Springer Nature; 2017.
Bakulin MK, Grudtsyna AS, Pletneva A. Biological fixation of nitrogen and growth of bacteria of the genus Azotobacter in liquid media in the presence of Perfluorocarbons. Appl Biochem Microbiol. 2007;4:399- 402.
Gupta AK. The complete technology book on biofertilizers and organic farming. Natio Insti of Indus Res Pres. India; 2004.
Paungfoo-Lonhienne C, Lonhienne TGA. Yeoh YK, Webb RI, Lakshmanan P, Chan CX, Lim PE, Ragan MA, Schmidt S, Hugenholtz P. A new species of Burkholderia isolated from sugarcane roots promotes plant growth. Microb Biotechnol. 2014;7(2):142-54.
Vadakattu G, Paterson J. Free-living bacteria lift soil nitrogen supply. Farming Ahead. 2006;116:40.
Sahoo, R.K., M.W. Ansari, T.K. Dangar, S. Mohanty N. Tuteja. Phenotypic and molecular characterisation of efficient nitrogen-fixing Azotobacter strains from rice fields for crop improvement. Protoplasma. 2014;251:511-523.
Venieraki A, Dimou M, Pergalis P, Kefalogianni I, Chatzipavlidis I, Katinakis P. The genetic diversity of culturable nitrogen-fixing bacteria in the rhizosphere of wheat. Microb Eco. 2011;61:277-285.
Boddey RM, de Oliveira OC, Urquiaga S, Reis VM, Olivares FL, Baldani VLD, DoEbereiner J. Biological nitrogen fixation associated with sugar cane and rice: contributions and prospects for improve-ment. Plant Soil. 1995;174:195-209.
Bandeppa S, Latha PC, Phule Amol S, Rajani G, Prasad Babu KV, Barbadikar Kalyani M, Chandrakala C, Prasad Babu MBB, Mandal PK, Sundaram RM. Isolation, identification and characterization of efficient free-living nitrogen-fixing bacteria from rice ecosystem. J. Rice Res. 2020;12(2):38-44.
Piper A, Das Saatgut VP. Darely Berlin, Hamburg, Germany; 1952.
Gupta PC. Seed vigour testing. In: Agarwal KP, editor. Handbook of seed testing. New Delhi; National Seed Corporation. 1993; 245-246.
Abdul-Baki AA, Anderson JD. Vigour determination in soybean seed by multiple criteria. Crop Sci. 1973;13:630-33.
Bal HB, Das S, Dangar TK, Adhya TK. ACC deaminase and IAA producing growth promoting bacteria from the rhizosphere soil of tropical rice plants. J. Basic Microbiol. 2013;53:972-984.
Bal HB, Adhya TK. Alleviation of submergence stress in rice seedlings by plant growth-promoting rhizobacteria with ACC deaminase activity. Front. Sustain. Food Syst. 2021;5:606158.
Garcia-Lemos AM, Grobkinsky DK, Saleem Akhtar S, Nicolaisen MH, Roitsch T, Nybroe O, Veierskov B. Identification of root-associated bacteria that influence plant physiology, increase seed germination, or promote growth of the christmas tree species Abies nordmanniana. Front. Microbiol. 2020;11: 566613.
Singh YR, Khunjamayum R, Nongthombam A, Chanu TP, Devi KM, Asem RS. Plant growth and grain yield production of black rice as influenced by Ochrobactrum intermedium AcRz3, an endophyte associated with medicinal plant. Crop Res. 2018;53:183-191.
Vidhyasri MS, Gomathi V, Siva Kumar U. Plant growth promotion of rice as influenced by Ochrobactrum sp. (MH685438) an rhizospheric bacteria associated with Oryzae sativa. Int. J.Curr. Microbiol. App. Sci. 2019;8(5):901-909.
Gholamalizadeh R, Khodakaramian G, Ebadi AA. Assessment of rice associated bacterial ability to enhance rice seed germination and rice growth promotion. Braz. Arch. Biol Technol. 2017;60:1- 13.
Nevita T, Sharma GD, Pandey P. Composting of rice-residues using lingocellulolytic plant-probiotic Stenotrophomonas maltophilia, and its evaluation for growth enhancement of Oryza sativa L. Environmental Sustainability. 2018;1:185-196.
Cassan F, Perrig D, Sgroy V, Masciarelli O, Penna C, Luna V. Azospirillum brasilense Az39 and Bradyrhizobium japonicum E109 promote seed germination and early seedling growth, independently or co-inoculated in maize (Zea mays L.) and soybean (Glycine max L.). Eur. J. Soil. Biol. 2009;45:28-35.
Zhao L, Xu Y, Lai XH, Shan C, Deng Z, Ji Y. Screening and characterization of endophytic Bacillus and Paenibacillus strains from medicinal plant Lonicera japonica for use as potential plant growth promoters. Braz. J. Microbiol. 2015;46: 977-89.
Zahir ZA, Arshad A, Frankenberger WT. Plant growth promoting rhizobacteria: application and prospectives in agriculture. Adv. Agron. 2004;81:97-168.
Muthukumarasamy R, Cleenwerck I, Revathi G, Vadivelu M, Janssens D, Hoste B, Gum KU, Ki-Do P, Son CY, Sa T, Caballero-Mellado J. Natural association of Gluconacetobacter diazotrophicus and diazotrophic Acetobacter peroxydans with wetland rice. Syst. Appl. Microbiol. 2005; 28(3):277-286.
Govindarajan M, Balandreau J, Kwon SW, Weon HY, Lakshminarasimhan C. Effects of inoculation of Burkholderia vietnamensis and related endophytic diazotrophic bacteria on grain yield of rice. Microbiol. Ecol. 2008;55:2-37.
Silva R, Filgueiras L, Santos B, Coelho M, Silva M, Estrada-Bonilla G, Vidal M, Baldani JI, Meneses C. Gluconacetobacter diazotrophicus changes the molecular mechanisms of root development in Oryza sativa L. growing under water stress. Int. J. Mol. Sci. 2020;21:333.
Schultz N, Pereira W, de Albuquerque Silva P, Baldani JI, Boddey RM, Alves BJR, Urquiaga S, Reis VM. Yield of sugarcane varieties and their sugar quality grown in different soil types and inoculated with a diazotrophic bacteria consortium. Plant Production Science. 2017;1-9.
Dal Cortivo C, Barion G, Visioli G, Mattarozzi M, Mosca G, Vamerali T. Increased root growth and nitrogen accumulation in common wheat following PGPR inoculation: assessment of plant-microbe interactions by ESEM. Agric. Ecosyst. Environ. 2017;247:396-408.
De Souza R, Ambrosini A, Passaglia LMP. Plant growth-promoting bacteria as inoculants in agricultural soils. Genet. Mol. Biol. 2015;38:401-419.
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