Molecular Characterization of Early Maturing Sugarcane Clones Using Microsatellite Markers
International Journal of Plant & Soil Science,
Sugarcane is an important cash crop of the entire world including India. It alone contributes to 75% of the worldwide sugar trade. Molecular markers are powerful tools and provide the basis for the estimation of genetic variability to start reasonable breeding program. Microsatellite markers have unique ability to determine the extent of genetic divergence among sugarcane genotypes. The objective of this study was to evaluate the genetic divergence of 12 early maturing sugarcane clones using 11 SSR markers. A total 55 alleles were found during the amplification of the primers out of which 21 alleles were found unique and 34 alleles were shared. The number of shared alleles per locus ranged from two out of five alleles in the case of primer NKS 1 and nine out of ten alleles in NKS 34. Similarly no. of unique alleles per locus ranged from one out of ten alleles in NKS 34, three out of six alleles in NKS 57. The primer pairs NKS 1 and NKS 8 generated considerably greater percentage of unique alleles. The PIC values revealing allelic diversity and frequency among the genotypes varied from 0.034 in case of NKS 48 to 0.778 in case of NKS 9 with an average of 0.549. Pair-wise combinations of CoSe15451 and CoSe15452 showed the highest similarity with the value of similarity coefficient (0.890). The lowest value of similarity coefficient was found to be 0.490 in the pair CoBln15501 and CoSe01421. The dendogram based on SSR marker analysis grouped the 12 sugarcane clones into four clusters which shows the CoSe15451 and CoSe15452 clones had maximum similarity and CoBln15501 and CoSe15452 clones had maximum diversity between each other.
- microsatellite marker
How to Cite
Suman SK, Kumar M, Kumar R, Kumar A, Singh D, Kumar A. Assessment of genetic diversity in inbred lines of maize (Zea mays L.) and its relationship with heterosis. International journal of Chemical Studies. 2020a;8(4):2917-2920.
Chen C, Yu Q, Hou S, Li Y, Eustice M, Skelton RL, Feng Y. Construction of a sequence-tagged high-density genetic map of papaya for comparative structural and evolutionary genomics in brassicales. Genetics. 2007;177(4):2481-2491.
Singh RK, Singh P, Misra P, Singh SP, Singh SB. STMS marker for tagging high sugar genes in sugarcane. Sugar Tech. 2005;7(2-3):74-76.
Pan YB. Highly polymorphic microsatellite DNA markers for sugarcane germplasm evaluation and variety identity testing. Sugar Tech. 2006;8(4):246-256.
Hoisington D. Laboratory protocol. Mexico, DF: CIMMYT applied molecular genetics laboratory; 1992.
Suman SK, Kumar M, Sharma VK, Kumar R. A modified and efficient CTAB genomic DNA extraction method from maize leaf for PCR analysis. Journal of Pharmacognosy and Phytochemistry. 2020;9(4):3418- 3420.
Anderson JA, Churchil GA, Autrique JE, Tanksley SO, Sorrels ME. Optimizing parent selection for genetic linkage maps. Genome. 1993;36:181-186.
Oliveira KM, Pinto LR, Marconi TG, Mollinari M, Ulian EC, Chabregas SM, Souza AP. Characterization of new polymorphic functional markers for sugarcane. Genome. 2009;52(2): 191-209.
Khan FA, Afzal A, Ijaz A, Ijaz U. Diversity analysis of sugarcane genotypes by microsatellite (SSR) markers. International Journal of Biotechnology and Molecular Biology Research. 2013;4(7):105-110.
Liu YQ, Yong B, Pan P. Highly polymorphic microsatellite DNA markers for sugarcane germplasm evaluation and variety identity testing. Sugar Tech. 2011;13(2):129-136.
Santosh M, Liu SJ, Tsunogae T, Li JH. Paleoproterozoic ultrahigh-temperature granulites in the North China Craton: implications for tectonic models on extreme crustal metamorphism. Precambrian Research. 2012;222:77- 106.
Sarath Padmanabhan TS, Hemaprabha G. Genetic diversity and population structure among 133 elite genotypes of sugarcane (Saccharum spp.) for use as parents in sugarcane varietal improvement. 3 Biotech. 2018;8(8):1-12.
Ali A, Pan YB, Wang QN, Wang JD, Chen JL, Gao SJ. Genetic diversity and population structure analysis of Saccharum and Erianthus genera using microsatellite (SSR) markers. Scientific Reports. 2019;9(1):1-10.
Cordeiro GM, Pan YB, Henry RJ. Sugarcane microsatellites for the assessment of genetic diversity in sugarcane germplasm. J. of Plant Sci. 2003;165:181-189.
Khan Q, Qin Y, Guo DJ, Zeng XP, Chen JY, Huang YY, Li YR. Morphological, agronomical, physiological and molecular characterization of a high sugar mutant of sugarcane in comparison to mother variety. Plos one. 2022;17(3): e0264990.
Huang HR, Gao YJ, Malviya MK, Verma KK, Solanki MK, Huang YX, Xu L. Genetic diversity analysis of sugarcane (Saccharum spp. hybrids) among high-sucrose clones of GT series and commonly used parents by using microsatellite markers in Guangxi, China. Sugar Tech. 2022;24(2):397-407.
Singh RK, Singh RB, Singh SP, Sharma ML. Identiﬁcation of sugarcane microsatellites associated to sugar content in sugarcane and transferability to other cereal genomes. Euphytica. 2011;182(1): 335–354.
Abstract View: 30 times
PDF Download: 18 times