Screening of Gene Based Markers with 23 Genotypes in Tomato (Solanum lycopersicum L)
K. Sushma *
Department of Vegetable Science, Sri Konda Laxman Telangana State Horticultural University (SKLTSHU), Rajendranagar, Hyderabad - 500 030, Telangana, India.
P. Saidaiah
College of Horticulture, Sri Konda Laxman Telangana State Horticultural University (SKLTSHU), Rajendranagar, Hyderabad - 500 030, Telangana, India.
Harikishan Sudini
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad – 502 324, Telangana, India.
A. Geetha
College of Agriculture, Professor Jayashankar Telangana State Agricultural University (PJTSAU), Palem – 509 215, Nagarkurnool District, Telangana, India.
K. Ravinder Reddy
College of Horticulture, Sri Konda Laxman Telangana State Horticultural University (SKLTSHU), Rajendranagar, Hyderabad - 500 030, Telangana, India.
*Author to whom correspondence should be addressed.
Abstract
The investigation was conducted at SKL Telangana State Horticultural University, Rajendranagar in Kharif, 2018 using 23 tomato genotypes. Screening with Ty-1 (P6-6) marker, only one genotype i.e. AVTO-1219 showed resistant band. Screening with Ty-2 marker, only 2 genotypes viz., AVTO-1219 and AVTO-9804 showed resistant bands. Screening with Ty-1 (SSR 47) marker, 3 genotypes i.e. AVTO-1219, AVTO-9803 and AVTO-9804 showed resistant bands. Using Fw-Z 1063 marker, 10 genotypes viz., EC-615055, EC-620428, AVTO-1219, EC-620378, EC-620389, EC-620394, EC-620422, EC-631369, EC-620503 and AVTO-9803 showed resistant bands. At molecular level, these ten genotypes showed resistant bands and these genotypes contain I-2 genes.
Keywords: Genetic diversity, gene specific primers, fusarium wilt, tomato yellow leaf curl virus and tomato spotted wilt virus.