Phytochemical and Thin Layer Chromatographic Analysis of Chloroform and Methanol Extracts of Azadirachta indica and Eucalyptus globulus Leaves
Nitin Vitthal Gurav
*
Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra, India.
Rajendra Marotrao Gade
Director of Instruction, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra, India.
Rupeshkumar Jagannath Choudhari
Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra, India.
*Author to whom correspondence should be addressed.
Abstract
The study focuses on phytochemical screening of Azadirachta indica and Eucalyptus globulus leaf extracts in chloroform and methanol solvents. The antibacterial compounds found in both leaf extracts of Azadirachta indica and Eucalyptus globulus plants were investigated using phytochemical studies. The extracts contained flavonoids, terpenoids, tannins, alkaloids, saponins, and phenolic chemicals, according to preliminary phytochemical screening. The solvent systems of hexane: ethyl acetate (1:1) and toluene: ethyl acetate (97:3) yielded the most chemicals from chloroform and methanolic extracts of A. indica and E. globulus plants, respectively. On TLC plates, these chemicals were separated, resulting in the discovery of different spots in both leaf extracts. The Rf values of chloroform extract of A. indica run under Hexane: Ethyl acetate (1:1) solvent system was 0.05, 0.11, 0.52, 0.58, 0.74, 0.82, 0.88, and 0.94, respectively, while Rf values of methanol leaf extract of E. globulus run under Toluene: Ethyl acetate (97:3) solvent system was 0.03, 0.07, 0.12, 0.25, 0.37, 0.43, 0.56, 0.62, 0.75, 0.81, 0.87, 0.88 and 0.94 respectively. The results of the investigation will be used to confirm the proper identification of A. indica and E. globulus crude plant extracts. The optimum solvents for extracting antibacterial components from A. indica and E. globulus leaves were chloroform and methanol.
Keywords: Azadirachta indica, Eucalyptus globules, phytochemical analysis, extraction, TLC, compounds, chloroform, methanol extract