Rooting Hormone and Substrate Effects on Mini-Cloned Mulberry (Morus indica)
Issue: 2023 - Volume 35 [Issue 20]
Department of Sericulture, Forest College and Research Institute,TamilNadu Agricultural University, India.
Susikaran S. *
Directorate of Open and Distance Learning, Tamil Nadu Agricultural University, India.
Shandeep S. G.
Department of Nematology, Tamil Nadu Agricultural University, India.
Haran M. S. R.
Department of Entomology, Tamil Nadu Agricultural University, India.
Department of Nematology, Tamil Nadu Agricultural University, India.
Department of Plantation and Spice crops, Tamil Nadu Agricultural University, India.
*Author to whom correspondence should be addressed.
Manipulation of propagation is a common practice in many commercial plant to obtain healthy and resistance plant for good yield. Many propagation techniques such as cutting, grafting, layering were evolved focusing on low budget production of high quality planting materials. An experiment was conducted to focus on standardizing a sound protocol for mini clonal propagation of M. indica variety V1. Among different concentrations of growth hormones evaluated, apical cuttings treated with IBA at 3000 ppm recorded significantly higher sprouting per cent (82.39%), rooting per cent (63.27%), survival per cent (68.80%), root length (22.22 cm), shoot length (22.05 cm), number of roots (14.40) and number of leaves (18.30) followed by NAA at 4000 ppm. Besides that, various rooting medium used, Soil: Coir pith: FYM (1:1:1) registered superior in survival per cent (14.26%), sprouting per cent (67.50%), rooting per cent (38.77%) and number of roots (12.45) whereas Soil: Sand: Vermicompost (1:1:1) registered maximum performance in shoot length (16.98 cm), root length (16.42 cm) and number of leaves (14.18). Hence it was clearly evident that IBA at 3000 ppm treated plants raised under Soil: Coir pith: FYM medium suitable for mass multiplication of M. indica.
Keywords: Mulberry, mini clone, rooting hormone, rooting media
How to Cite
Rahdari P, Mohanna M, Asadi A. Effect of zinc sulfate and IBA and NAA on rooting of semihardwood cuttings of Aralia spp. and its’ bio-environment effect. Journal of Sciences and Techniques in Natural Resources. 2010;5(1):95-103.
Parvez S, Ud-Din SN, Shafi S, Maqbool S, Bhat IA. To study the performance of summer raised rooted saplings in field at the end of different seasons; 2023.
Choudhary R, Malik SK, Chaudhury R, Rao AA. Optimized Recovery of Cryostored Dormant Buds of Mulberry Germplasm. Plants. 2023;12(2):225.
Tikader A, Vijayan K. Assessment of biodiversity and strategies for conservation of genetic resources in mulberry (Morus spp.). Tree and Forest Biodiversity. Bioremediation, Biodiversity and Bioavailability. 2010;4(1):15-27.
Samami R, Seidavi A, Eila N, Moarefi M, Ziaja DJ, Lis JA, Cappai MG. Production performance and economic traits of silkworms (Bombyx mori L., 1758) fed with mulberry tree leaves (Morus alba, var. Ichinose) significantly differ according to hybrid lines. Livestock Science. 2019;226:133-137.
Saraçoğlu O, Erdem SÖ, Çekiç Ç, Yıldız K. Application of new vegetative propagation methods for black mulberry. Uludağ Üniversitesi Ziraat Fakültesi Dergisi. 2016;30:624-627.
Vijayan K, Tikader A, da Silva JAT. Application of tissue culture techniques for propagation and crop improvement in mulberry (Morus spp.). Tree For Sci Biotechnol. 2011;5(1):1-13.
Hartmann HT, Kester DE, Devies FT and RL Geneve,. Plant Propagation: Principles and Practices. 7th ed. Prentice-Hall. Englewood Cliffs, New Jersey. 2002; 363-365.
Parthiban KT, Seenivasan R. Forestry technologies-a complete value chain approach. Scientific Publishers; 2002.
Parthiban KT, Subbulakshmi V, Paramathma M, Devanand PS. Dardizing cutting size for clonal multiplication of Jatropha curcas. Agricultural Science Digest-A Research Journal. 2014;34(2):147-150.
Husen A, Iqbal M, Siddiqui SN, Sohrab SS, Masresha G. Effect of indole-3-butyric acid on clonal propagation of mulberry (Morus alba L.) stem cuttings: Rooting and associated biochemical changes. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 2014;87:161-166.
Ahalya BN. Genotype Environmental Interaction for Growth and Yield Parameters of Tree Mulberry Genotypes in Different Seasons and Bioassay Studies of Silkworm Bombyx mori L. Mysore Journal of Agricultural Sciences. 2014;56(1).
Sajeevan RS, Singh SJ, Nataraja KN, Shivanna MB. An efficient in vitro protocol for multiple shoot induction in mulberry, Morus alba L variety V1. Int. Res. J. Plant Sci. 2011;2(8):254-261.
Mogili T, Rajashekar K, Balakrishna R, Kamble CK. Development of protocol for raising mulberry saplings from soft wood and semi-soft wood cuttings. Advances in Plant Sciences. 2011:24(2):443-446.
Kiruthika C, Susikaran S, Parthiban KT, Krishnamoorthy S V. Role of Auxins on growth of apical shoot cuttings of mulberry (Morus indica L.) using Mini clonal technology. IJCS. 2011;8(4):1896-1899.
Wiesman Z, Riov J, Epstein E. Comparison of movement and metabolism of indole‐3‐acetic acid and indole‐3‐butyric acid in mung bean cuttings. Physiologia Plantarum. 1988;74(3):556-560.
Sun J, Li H, Chen H, Wang T, Quan JE, Bi H. The Effect of Hormone Types, Concentrations, and Treatment Times on the Rooting Traits of Morus ‘Yueshenda 10’Softwood Cuttings. Life. 2023;13(4):1032.
Heloir MC, Kevers C, Hausman JF, Gaspar T. Changes in the concentrations of auxins and polyamines during rooting of in-vitro-propagated walnut shoots. Tree Physiology. 2023;16(5):515-519.
Husen A, Pal M. Effect of serial bud grafting and etiolation on rejuvenation and rooting cuttings of mature trees of Tectona grandis Linn. f. Silvae Genetica, 2003;52(2):84-87.
Koyuncu F, Senel E. Rooting of Black Mulberry (Morus nigra L.) hardwood cuttings. J of fruit and ornament plant Res. 2003;11:53-57.
Rani S, Sharma A, Wali VK, Bakshi P, Ahmed S. The standardization of method and time of propagation in guava (Psidium guajava). Indian Journal of Agricultural Sciences. 2015;85(9):38-45
Reddy KV, Reddy CP, Goud PV. Effect of Auxins on the rooting of fig (Ficus carica L.) hardwood and semi hardwood cuttings. Indian Journal of Agricultural Research. 2008;42(1):75-78.
Abdullah ATM, Hossain MA, Bhuiyan MK. Clonal propagation of guava (Psidium guajava Linn.) by stem cutting from mature stockplants. Journal of Forestry Research. 2006;17:301-304.
Sukhjit K. Effect of different treatments of Indole3-butyric acid (IBA) on the rooting and growth performance of hardwood cuttings of Peach (Prunus persica L.).Agric. Sci. Digest. 2015;35(1):41-45.
Singh KK, Choudhary T, Kumar A. Effect of various concentrations of IBA and NAA on the rooting of stem cuttings of mulberry (Morus alba L.) under mist house condition in Garhwal Hill region. Indian Journal of Hill Farming, 2014;27(1):125-131.
Sinha PS, Gangwar SK, Singh BD, Jaiswal J, Griyaghey UP. Evaluation of some elite Mulberry (Morus alba L.) varieties and NPK levels under partially irrigated conditions from sericulture view point. Indian Journal of Agricultural Research. 2001; 35(2):71-78.
Husen A. Rejuvenation and adventitious rooting in coppice-shoot cuttings of Tectona grandis as affected by stock-plant etiolation. American Journal of Plant Sciences. 2011;2(3):370-374.
Husen A. Clonal multiplication of teak (Tectona grandis) by using moderately hard stem cuttings: effect of genotypes (FG1 and FG11 Clones) and IBA treatment. Advances in Forestry Letters. 2013;2(2):14-19.
Kalyoncu IH, Ersoy N, Yılmaz M, Aydın M. Effects of humidity level and IBA dose application on the softwood top cuttings of white mulberry (Morus alba L.) and black mulberry (Morus nigra L.) types. African Journal of Biotechnology. 2009;8(16).
Habibi P, de Sa MFG, da Silva ALL, Makhzoum A, da Luz Costa J, Borghetti IA, Soccol CR . Efficient genetic transformation and regeneration system from hairy root of Origanum vulgare. Physiology and molecular biology of plants. 2016;22:271-277.
Leakey RRB, Chapman VR, Longman KA. Physiological studies for tropical tree improvement and conservation. Factors affecting root initiation in cuttings of Triplochiton scleroxylon K. Schum. Forest Ecology and management. 1982;4(1):53-66.
Singh KK, Rawat JMS, Tomar YK. Influence of IBA on rooting potential of torch glory Bougainvillea glabra during winter season. Journal of Horticultural Science & Ornamental Plants. 2011;3(2):162-165.
Singh KK, Krishan C, Singh KKK. Propagation of citrus species through cutting: A review. Journal of Medicinal Plants Studies. 2018;6(1):167-172.
Packialakshmi M, Jude Sudhagar R. Standardisation of rooting hormone in mini clonal technology of Tectona grandis. International Journal of Chemical Studies. 2019;7(3):4398-4401
Ullah T, Wazir FU, Ahmad M, Analoui F, Khan MU. A breakthrough in guava (Psidium guajava L.) propagation from cutting. Asian J. Plant Sci. 2005;4: 238–243.
Pallavi D, Sharma GL, Naik KL. Effect of IBA and NAA on rooting and growth of mulberry cuttings. Int J Curr Microbiol App Sci. 2018;7(11):305-308
Singh KK. Effect of auxins and rooting media on rooting in stem cutting of mulberry (Morus nigra L.). The Pharma Innovation Journal. 2018;7(11):12-15.
Ghatnatti, SA. Studies on propagation of Durantha plumeri Jacq. Var Goldiana by stem cuttings with growth regulators under mist. M.Sc.(Agri.) Thesis, Univ. Agric. Sci;1997.
Baroudi H, Makhoul G, Mahfoud H. The effect of IBA (Indole-3-Butyric Acid) dose applications on rooting of hardwood and softwood top cuttings of white mulberry (Morus alba L.) and black mulberry (Morus nigra L.). Syrian Journal of Agricultural Research. 2017;5(2):2334.
Galavi M, Ali Karimian M, Mousavi SR. Effects of Different Auxin (IBA) Concentrations and Planting-Beds on Rooting Grape Cuttings (Vitis vinifera). Annual Review & Research in Biology. 2013;3(4):517-523.
Kumar P. Genetic evaluation, Growth characterization and clonal propagation studies in Melia dubia Cav. Ph.D. Thesis, Tamil Nadu Agricultural University, Coimbatore; 2011.
Wahab F, Nabi G, Ali N, Shah M. Rooting response of semi hard wood cuttings of guava (Psidium guajava L.) to various concentrations of different auxins. Online Journal of Biological Sciences. 2001;1(4):184-87.
Siddiqui MI, Hussain SA. Effect of indole butyric acid and types of cuttings on root initiation of Ficus hawaii. Sarhad Journal of Agriculture. 2007;23(4):919.
Alam R, Rahman KU, Ilyas M, Ibrahim M, Rauf MA. Effect of Indole Butyric acid concentrations on the rotting of Kiwi cuttings. Sarhad Journal of Agriculture. 2007;23(2):293.
Husen A. Clonal propagation of Dalbergia sissoo Roxb. by softwood nodal cuttings: effects of genotypes, application of IBA and position of cuttings on shoots. Silvae Genetica. 2004;53(1-6):50-55.
Guleria V, Vashisht A. Rejuvenation and adventitious rooting in shoot cuttings of Tectona grandis under protected conditions in new locality of Western Himalayas. Universal Journal of Plant Science. 2014;2(6):103-1006.