A Simplified, Efficient and Rapid DNA Extraction Protocol from Rice Grains and Leaf

Pradeep Kumar *

Department of Agricultural Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, U.P. (India)-250110 (Presently Working, ICAR-Indian Grassland and Fodder Research Institute, Jhansi U.P. 284003), India.

Alamgir

Department of Agricultural Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, U.P., 250110, India.

Simran Kirti

Department of Agricultural Biotechnology and Molecular Biology, Dr. Rajendra Prasad Central Agriculture University, Pusa Samastipur (Bihar) 848125, India.

Ritu Rani

Department of Agricultural Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, U.P., 250110, India.

Pusphendra Singh

Department of Agricultural Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, U.P., 250110, India.

Kiran

Department of Biochemistry, Dr. Rajendra Prasad Central Agriculture University, Pusa Samastipur (Bihar) 848125, School of Basic and Applied Science, Galgotias University, Greater Noida (Uttar Pradesh), India.

*Author to whom correspondence should be addressed.


Abstract

DNA extraction is very complicated with different plant species because the presence of secondary metabolites that hinder with DNA isolation processes and application like DNA restriction, Gene amplification, as well as gene cloning. A simple method for preparation of rice genomic DNA was developed. The current study expressed comparatively rapid, cost-effective, less time consuming, methods for DNA extraction from seed, young leaves and old leaves of rice genotypes without using liquid nitrogen, phenol, and β–mercaptoethanol in extraction buffer. Using this method, high quality and quantity of genomic DNA was get from 0.5 g of rice seed, young leaves and 75 days old leavesand extracted total nucleic acids (genomic DNA) were amplified by employing single sequence repeats (SSR) or microsatellite markers, which produced reproducible results.This protocol resolves the problems of DNA degradation, contamination, and low yield due to binding and/or coprecipitation with starches and polysaccharides.

Keywords: DNA isolation, PCR, Oryza sativa, molecular marker, DNA extraction, molecular breeding


How to Cite

Kumar, Pradeep, Alamgir, Simran Kirti, Ritu Rani, Pusphendra Singh, and Kiran. 2023. “A Simplified, Efficient and Rapid DNA Extraction Protocol from Rice Grains and Leaf”. International Journal of Plant & Soil Science 35 (21):517-24. https://doi.org/10.9734/ijpss/2023/v35i214005.