Exploitation of Wheat Genome through Triticale x Wheat Cross for Improvement and Identification of Large Anther
Vikrant Khare
*
Division of Nuclear Agriculture and Biotechnology, Bhabha Atomic Research Centre, Mumbai, India.
Puja Srivastava
Department of Plant Breeding and Genetics, Punjab Agriculture University, Ludhiana, India.
Achla Sharma
Department of Plant Breeding and Genetics, Punjab Agriculture University, Ludhiana, India.
N. S. Bains
Department of Plant Breeding and Genetics, Punjab Agriculture University, Ludhiana, India.
*Author to whom correspondence should be addressed.
Abstract
Triticale is a man-made cereal crop that combines the rye and wheat genome in a single crop. Rye is a cross pollinated cereal crop that has large anther size in comparison of wheat. To improve wheat anther size, cross with triticale is a noble practice to combine the A, B, D and R genome. In the current investigation wheat (AABBDD) were crossed with triticale (AABBRR) with the help of Ph suppressor Chinese Spring. Subsequently triticale wheat derivative lines populations were developed. The BC2F7 generations of the derived population were planted in alpha lattice design to further evaluation. With the help of fixative, the fresh spikes were collected from individual derivative lines to measure anther the size. The anther size was measured in millimeter by the help of ocular micrometer. Twenty-two triticale wheat derivative lines were identified with grater anther size in comparison of triticale anther. The TWD26 (5.7mm) has larger anther size followed by TWD18 (5.4mm) and TWD36 (5.3mm). These derivative lines may use as a donor parent in hybrid wheat breeding programme.
Keywords: Triticale, out-crossing, wheat, rye, anther size