Chemical Characterization of Supercritical Fluid Extract (SFE) of Ailanthus excelsa and Estimation of Total Phenols and Flavonoids in SFE
Amaresh, Y. S *
Directorate of Research, University of Agricultural Sciences, Raichur, Karnataka, India.
Kavyasri, M
Department of Plant Pathology, University of Agricultural Sciences, Raichur, Karnataka, India.
Ashwathanarayana D. S
Department of Plant Pathology, University of Agricultural Sciences, Raichur, Karnataka, India.
Raghavendra, B. T
Department of Plant Pathology, University of Agricultural Sciences, Raichur, Karnataka, India.
Sharanagoudar Hiregoudar
Department of Processing and Food Engineering, University of Agricultural Sciences, Raichur, Karnataka, India.
*Author to whom correspondence should be addressed.
Abstract
Ailanthus excelsa which is usually called as tree of heaven is known to possess anti- fungal activity. The bio constituents such as flavonoids and phenols which are present in the leaves of Ailanthus excelsa is known to possess anti- bacterial, anti- fungal properties. This experiment was conducted to study the fungicidal activity of Ailanthus excelsa and its characeterization. Results revealed that the supercritical fluid extraction was effective at 60⁰C of temperature and 225 bar pressure with solvent flow rate of 5 g/min and caron di- oxide flow rate at 20 g/min. The GC-MS analysis showed that forty different constituents were present in the A. excelsa. Among which, Ricinoleic acid, Diethyl phthalate, 1.63 Hexadecanoic acid, methyl ester and 9- octadecenoic acid (Z)-, methyl ester were found to impart anti- fungal activity. The total phenols and flavonoids was 19.65 mgGAEg-1 and 40.68 mgGAEg-1 respectively was found in the SFE extract of A. excelsa.
Keywords: Supercritical fluid extract, characterization, ailanthus excels, antifungal activities, anti- fungal properties, ayurvedic formulations