Comparative Evaluation of In-vitro Bioefficacy of Microbial Bioagents and Novel Chemical Compounds against Fusarium oxysporum f.sp. Cubense

Anisha Baruah

Biocontrol Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, India.

Popy Bora

Biocontrol Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, India and AAU-Assam Rice Research Institute, Titabor, Assam Agricultural University, Jorhat, 785013, India.

Ankita Saikia *

Biocontrol Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, India.

Trishna Taye *

Biocontrol Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, India.

Bishal Saikia

Biocontrol Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, India.

Parveen Khan

AAU-Zonal Research Station, Assam Agricultural University, Karimganj-788712, India.

Anwesha Sharma

Biocontrol Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, India.

*Author to whom correspondence should be addressed.


Abstract

The Assam and North-eastern regions of India recognized as diverse repositories of wild and cultivated banana cultivars, confront a significant threat to banana varieties, Malbhog due to Fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense (Foc). This study investigates the in-vitro bioefficacy of indigenous bioagents and new-generation chemical compounds against Foc to develop an integrated module subsequently, in line with the worldwide pursuit of environmentally conscientious and high-performance agricultural practices.In this study, four bioagents viz., Bacillus vallismortis, Bacillus amyloliquefaciens, Trichoderma harzianum, and Talaromyces pinophilus were screened in-vitro against Foc revealed the highest efficacy of B. vallismortis with 68.22 % mycelial growth inhibition of Foc followed by B. amyloliquifaciens with (60.05%) and the least inhibition was exhibited by T.pinophilus with (50.05%).To identify new generation chemical compounds, namely Propiconazole, Azoxystrobin, Tebuconazole + Trifloxystrobin were tested with a standard check Carbendazim wherein all the chemicals significantly inhibited the mycelial growth of the pathogen over control with Tebuconazole + Trifloxystrobin combination fungicide showing highest percent inhibition of 94% at 0.1% concentration followed by Carbendazim at 0.1%. Our study has identified potential microbial strains and chemical fungicides which can be further explored for the development and exploration of bio-fungicide and chemical fungicide-based biointensive integrated management of Fusarium wilt menace in malbhog banana in the region.

Keywords: Biocontrol, bio-fungicide, chemical fungicide, fusarium wilt, malbhog banana


How to Cite

Baruah , Anisha, Popy Bora, Ankita Saikia, Trishna Taye, Bishal Saikia, Parveen Khan, and Anwesha Sharma. 2024. “Comparative Evaluation of In-Vitro Bioefficacy of Microbial Bioagents and Novel Chemical Compounds Against Fusarium Oxysporum f.Sp. Cubense”. International Journal of Plant & Soil Science 36 (7):1-9. https://doi.org/10.9734/ijpss/2024/v36i74702.

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References

Zhang P, Whistler RL, BeMiller JN, Hamaker BR. Banana starch: Production, physicochemical properties, and digestibility—A review. Carbohydrate Polymers. 2005;59(4):443-458.

Singh S, Singh N, Kumar V, Datta S, Wani AB, Singh D, Singh J. Toxicity, monitoring and biodegradation of the fungicide carbendazim. Environmental Chemistry Letters. 2016;14(3):317-329.

FAOSTAT; 2021. DOI:www.faostat.fao.org

Sarma U, Govila VK, Yadav A. The traditional and therapeutic use of banana and its plant based delicacies in ethnic Assamese cuisine and religious rituals from Northeast India. Journal of Ethnic Foods. 2020;7(1):1-7.

Savani AK, Bhattacharyya A, Boro RC, Dinesh K, Jc NS. Exemplifying endophytes of banana (Musa paradisiaca) for their potential role in growth stimulation and management of Fusarium oxysporum f. spcubense causing panama wilt. Folia Microbiologica. 2021;66:317-330.

Kumari N, Damodaran T, Ahmad I, Rajan S, Shukla PK, Manoharan M, Kushwaha AK, Singh H, Gopal R, Kumari S, Yadav K, Bora P, Jha SK. Distribution and diversity of Fusarium oxysporum f. sp. cubense TR4 causing banana wilt in Sub-tropics of India and comparative analysis of TR4 specific molecular detection methods. Journal of Plant Biochemistry and Biotechnology. 2023;32(3):570-586.

Damodaran T, Rajan S, Muthukumar M, Gopal R, Yadav K, Kumar S, Jha SK. Biological management of banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 using antagonistic fungal isolate CSR-T-3 (Trichoderma reesei). Frontiers in Microbiology. 2020;11:595845.

Bora P, Gogoi S, Deshpande MV, Garg P, Bhuyan RP, Altaf N, Saha N, Borah SM, Phukon M, Tanti N, Saikia B, Ahmed SS, Borah SR, Dutta A, Sarmah BK. Rhizospheric Bacillus spp. Exhibit Miticidal Efficacy against Oligonychuscoffeae (Acari: Tetranychidae) of Tea. Microorganisms. 2023;11(11):2691.

Jangir M, Pathak R, Sharma S, Sharma S. Biocontrol mechanisms of Bacillus sp., isolated from tomato rhizosphere, against Fusarium oxysporum f. sp. lycopersici. Biological Control. 2018;123: 60-70.

Srivastava AK, Bora P. Multiple dimensions of agroecology in sustaining agriculture. Indian Farming. 2023;73(6):35-37.

The Gazette of India; 2020 (18/05/2020).

Bora P, Bora LC. Disease management in horticulture crops through microbial interventions: An overview. Indian Journal of Agricultural Sciences. 2020;90(8):1389-1396.

Damodaran T, Mishra M, Muthukumar M, Rajan S, Yadav K, Kumar A, Debnath P, Kumari S, Bora P, Gopal R, Kumar S. Secondary metabolite induced tolerance to Fusarium oxysporum f. sp. cubenseTR4 in banana cv. Grand Naine through in vitro bio-immunization: A prospective research translation from induction to field tolerance. Frontiers in Microbiology. 2023;14: 1233469.

Saikia K, Bora LC, Bora P, Hazarika H. Management of bacterial blight in rice (Oryza sativa) through combined application of endophytes and rhizosphere antagonist. Indian J Agric Sci. 2020;90(12): 2323-7.

Bora P, Bora LC, Bhuyan RP, Hashem A, Abd-Allah EF. Bioagent consortia assisted suppression in grey blight disease with enhanced leaf nutrients and biochemical properties of tea (Camellia sinensis). Biological Control. 2022;170: 104907.

Srivastava AK, Das AK, Jagannadham PTK, Bora P, Ansari FA., and Bhate, R. (2022). Bioprospecting microbiome for soil and plant health management amidst huanglongbing threat in citrus: a review. Frontiers in Plant Science. 2023;13: 858842.

Cheng S, Li TIAN, Ying-Ning ZOU, Qiang-Sheng WU, Kamil KUČA, Popy BORA. Molecular responses of arbuscular mycorrhizal fungi in tolerating root rot of trifoliate orange. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2020;48(2):558-571.

Chaudhary P, Agri U, Chaudhary A, Kumar A, Kumar G. Endophytes and their potential in biotic stress management and crop production. Frontiers in Microbiology. 2022;13:933017.

Taye T, Saikia B, Panging K. Biocontrol agents against post harvest decay in fruits and vegetables: A review. International Journal of Plant & Soil Science. 2023; 35(10):145-156.

Baruah A. Diversity of Fusarium oxysporumf.spcubense causing Fusarium wilt of Malbhog banana and assessment of cultures filtrates of bioagents and new generation fungicides.M.Sc Thesis, Assam Agricultural University (Jorhat, Assam) India; 2023.

Dennis C, Webster J. Antagonistic properties of species-groups of Trichoderma: II. Production of volatile antibiotics. Transactions of the British Mycological Society. 1971;57(1):41-8.

Utkhede RS, Rahe JE. Interactions of antagonist and pathogen in biological control of onion white rot. Phytopathology. 1983;73(6):890-893.

Vincent JM. Distortion of fungal hyphae in the presence of certain inhibitors. Nature. 1927;59:850.

Nene, YL, Thapliyal PN. Poisoned food technique. Fungicides in Plant Disease Control. 2000;15:531-533.

Snedecor GW. Cochran WG, Fuller JR. Métodosestadísticos (No. 04; QA276. 12, S5.). México: Continental; 1971.

Kaur PK, Kaur J, Saini HS. Antifungal potential of Bacillus vallismortis R2 against different phytopathogenic fungi. Spanish Journal of Agricultural Research. 2015;13(2):e1004-e1004.

Saikia A. Root endophytes from wild banana plant and their antagonistic potential against major pathogens of malbhog banana. M.Sc Thesis, Assam Agricultural University (Jorhat, Assam) India; 2022.

Ravi S, Sevugapperumal N, Nallusamy S, Shanmugam H, Mathiyazhagan K, Rangasamy A, Varagur Ganesan M. Differential bacterial endophytome in Foc‐resistant banana cultivar displays enhanced antagonistic activity against Fusarium oxysporum f. sp. cubense (Foc). Environmental Microbiology. 2022;24(6): 2701-2715.

Yadav K, Damodaran T, Dutt K, Singh A, Muthukuma M, Gopal R, Rajan S, Sharma PC. Effective biocontrol of banana fusarium wilt tropical race 4 by a bacillus rhizobacteria strain with antagonistic secondary metabolites. Rhizosphere. 2021;18: 100341.

Bora P, Saikia B, Rahman M, Ahmed SS, Chetia R, Rahman N, Nath BC,Raja WH. Enhancing the performance of chilli (Capsicum annuum) through twin role of plant growth promotion and disease suppressionvia Bacillus subtilis-based bioformulation. The Indian Journal of Agricultural Sciences. 2024;94(1):039-043.

Bora P, Bora LC. Microbial antagonist and botanicals mediated disease management in tea, Camellia sinensis (L.) O. Kuntze: An Overview. Crop Protection. 2021;148: 105711.

Tian Y, Zhao Y, Fu X, Yu C, Gao K, Liu H. Isolation and Identification of Talaromyces sp. Strain Q2 and Its Biocontrol Mechanisms Involved in the Control of Fusarium Wilt. Frontiers in Microbiology. 2021;12.

Mukherjee PK, Crutcher FK, Parich A, Schuhmacher R, Zeilinger S, Kenerley CM. A putative terpene cyclase, vir4, is responsible for the biosynthesis of volatile terpene compounds in the biocontrol fungus Trichoderma virens. Fungal Genetics and Biology. 2013;56:67-77.

Mishra A, Harshita AS, Khan JB, Tripathi UK, Ratan V, Trivedi S, Mishra A Verma A. In-vitro evaluation of systemic fungicides against Fusarium oxysporumf. sp. lycopersici and their compatibility with bioagents. Journal of Pharmacognosy and Phytochemistry. 2019;8(3): 3117-3123.

Niwas R, Chand G, Azad CS. In vitro evaluation of fungicides against growth of Fusarium oxysporum f. sp.cubense causing panama wilt disease of Banana. International Journal of Chemical Studies. 2020;8(1):191-194.

Somu R, Thammaiah N, Swamy, GSK Kulkarni, MS Devappa V. In vitro evaluation of fungicides against Fusarium oxysporumf. sp. cubense. International Journal of Plant Protection. 2014;7(1):221-224

Gupta RC, Crissman JW. Agricultural chemicals. In Haschek and Rousseaux's Handbook of Toxicologic Pathology, Academic Press. 2013;1349-1372.

Saikia B, Bora P, Taye T, Chetia R, Tabing R, Neog T, Nayak S. Biocontrol potential of Bacillus subtilis Lb22against fruit rot of King chilli, Capsicum chinense Jacq. Pest Management in Horticultural Ecosystems. 2022;28(2):167-173.

Ahmed S, Saikia B, Saikia A. Disease dynamics in fruit crops: Tracking the journey for better sustainability. International Journal of Innovative Horticulture. 2023;12(2):136-142.

Fan X, Huo X, Zhang X, Chen X, Qiao X, Zhang D. Isolation and yield optimization of lipopeptides from Bacillus subtilis Z‐14 active against wheat take‐all caused by Guamannomyces graminis var. tritici. Journal of Separation Science. 2021;44(4): 931-940.

Gomez KA, Gomez AA. Statistical procedures for agricultural research. John wiley& sons; 1984.