Comparative Evaluation of In-vitro Bioefficacy of Microbial Bioagents and Novel Chemical Compounds against Fusarium oxysporum f.sp. Cubense

Anisha Baruah

Biocontrol Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, India.

Popy Bora

Biocontrol Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, India and AAU-Assam Rice Research Institute, Titabor, Assam Agricultural University, Jorhat, 785013, India.

Ankita Saikia *

Biocontrol Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, India.

Trishna Taye *

Biocontrol Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, India.

Bishal Saikia

Biocontrol Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, India.

Parveen Khan

AAU-Zonal Research Station, Assam Agricultural University, Karimganj-788712, India.

Anwesha Sharma

Biocontrol Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, India.

*Author to whom correspondence should be addressed.


The Assam and North-eastern regions of India recognized as diverse repositories of wild and cultivated banana cultivars, confront a significant threat to banana varieties, Malbhog due to Fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense (Foc). This study investigates the in-vitro bioefficacy of indigenous bioagents and new-generation chemical compounds against Foc to develop an integrated module subsequently, in line with the worldwide pursuit of environmentally conscientious and high-performance agricultural practices.In this study, four bioagents viz., Bacillus vallismortis, Bacillus amyloliquefaciens, Trichoderma harzianum, and Talaromyces pinophilus were screened in-vitro against Foc revealed the highest efficacy of B. vallismortis with 68.22 % mycelial growth inhibition of Foc followed by B. amyloliquifaciens with (60.05%) and the least inhibition was exhibited by T.pinophilus with (50.05%).To identify new generation chemical compounds, namely Propiconazole, Azoxystrobin, Tebuconazole + Trifloxystrobin were tested with a standard check Carbendazim wherein all the chemicals significantly inhibited the mycelial growth of the pathogen over control with Tebuconazole + Trifloxystrobin combination fungicide showing highest percent inhibition of 94% at 0.1% concentration followed by Carbendazim at 0.1%. Our study has identified potential microbial strains and chemical fungicides which can be further explored for the development and exploration of bio-fungicide and chemical fungicide-based biointensive integrated management of Fusarium wilt menace in malbhog banana in the region.

Keywords: Biocontrol, bio-fungicide, chemical fungicide, fusarium wilt, malbhog banana

How to Cite

Baruah , Anisha, Popy Bora, Ankita Saikia, Trishna Taye, Bishal Saikia, Parveen Khan, and Anwesha Sharma. 2024. “Comparative Evaluation of In-Vitro Bioefficacy of Microbial Bioagents and Novel Chemical Compounds Against Fusarium Oxysporum f.Sp. Cubense”. International Journal of Plant & Soil Science 36 (7):1-9.


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