Natural Defense: The Inhibitory Effects of Endophytic Bacillus and Xylaria sp. against Rice Pathogens
Sushitha *
Department of Plant Pathology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala Agricultural University, 695 522, India.
A. Sajeena
Integrated Farming System Research Station, Thiruvananthapuram, Kerala Agricultural University, 695 002, India.
N. V. Radhakrishnan
Department of Plant Pathology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala Agricultural University, 695 522, India.
Joy Michal Johnson
Department of Plant Pathology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala Agricultural University, 695 522, India.
Swapna Alex
Department of Molecular Biology and Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala Agricultural University, 695 522, India.
R. Beena
Department of Plant Physiology, College of Agriculture, Vellayani, Kerala Agricultural University, Thiruvananthapuram 695 522, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: Endophytes living inside the plant tissues are known to play a crucial role in the physiology and developmental functioning of host plants. Rice is the major food crop that feeds half of the world population. But pest and diseases lead to drastic yield reduction in rice. Sustainable and eco-friendly disease management strategies are urgently needed to reduce the reliance on chemical pesticides and mitigate the environmental impact of intensive agriculture.The aim of the study was the selection of natural suppressors of the brown spot and sheath blight diseases in rice. In this study, three bacterial endophytes viz., Bacillus subtilis isolate PAL 11, B. altitudins isolate TSL-4 and B. altitudinis isolate KAM-11 and one endophytic fungal isolate Xylaria sp. isolate KTD-2 were tested for their potential to inhibit Bipolaris oryzae and Rhizoctonia solani under in vitro conditions.
Methodology: In vitro evaluation of endophytes against two major rice pathogens viz., Rhizoctonia solani and Bipolaris oryzae causing sheath blight and brown leaf spot respectively were tested by dual culture technique.
Results: All the tested bacterial and fungal endophytes significantly inhibited both the pathogens under in vitro conditions. Among the bacterial endophytes, B. subtilis isolate PAL-11 exhibited highest inhibition of both the pathogens. The mycelial inhibition of B. oryzae was 56.04 and 74.48 per cent respectively at 5 and 8 days after dual culturing whereas the inhibition of R. solani was 80.31 and 80.22 per cent respectively at 3 and 5 days after dual culturing. The fungal endophyte Xylaria sp. isolate KTD-2 exhibited 63.2 and 67.95 per cent inhibition of mycelial growth of B. oryzae at 5 and 8 days respectively after the pathogen inoculation. The mycelial growth of R. solani was also significantly inhibited (54.95 %) by the fungal endophyte at 5 days after pathogen inoculation.
Conclusion: This research highlights the potential role of endophytes in sustainable agriculture and suggests that integrating beneficial microbes into rice disease management strategies could help enhance plant health, increase crop yields, and contribute to global food security.
Keywords: Endophytes, bacillus sp., xylaria sp., brown spot, sheath blight