In vitro and In-vivo Evaluation of Different Fungicides against Alternaria brassicae Causing Alternaria Blight Disease of Mustard
Mariya Ansari *
Department of Mycology and Plant Pathology, Banaras Hindu University, Varanasi-221005, U.P, India.
Amarendra Kumar
Department of Plant Pathology, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India.
Rehan Reza
Department of Soil Science and Agricultural chemistry, BAU, Sabour, Bhagalpur, Bihar, India.
Shweta Meshram
School of Agriculture, LPU, 144412, Punjab, India.
*Author to whom correspondence should be addressed.
Abstract
Mustard is the second most important oilseed crop in India after groundnut and contributes about 35% in the total vegetable oil production. Mustard crop is sensitive to numerous abiotic and biotic stresses, which affects the production and productivity drastically. Alternaria blight disease of mustard is one the most destructive disease which affects all growth stages and cause huge economical loss. Considering the severity and significance of damage caused by this disease, an in vitro and in vivo evaluation of eight fungicide was done against A. brassicae. It was found that Propiconazole 25 EC showed complete mycelial growth inhibition (100 %) at all the three concentrations i.e., 100, 200 and 300 ppm whereas, Tebuconazole 25.9 EC recorded complete mycelial growth inhibition at 200 and 300 ppm. Among the tested fungicides, the minimum percent disease severity and AUDPC on leaves was observed in crops sprayed with Azoxystrobin 12.5% + Tebuconazole 12.5% SC followed by Tebuconazole 50% + Trifloxystrobin 25% WG and Azoxystrobin 235 SC at 40, 55 and 70 days after spraying. Further, maximum seed yield was recorded in Azoxystrobin 12.5% + Tebuconazole 12.5% SC at 1345.49Kg/ha followed by Azoxystrobin 12.5% at 1265.63kg/ha.
Keywords: Alternaria blight disease, fungicide, poisoned food technique, AUDPC, correlation coefficient, disease severity