International Journal of Plant & Soil Science

This study investigated seed viability, water imbibition behavior, and desiccation tolerance of Gymnema sylvestre R.Br. to evaluate its potential for propagation and long-term storage. Tetrazolium (TZ) staining tests at varying concentrations (0.25%, 0.5%, and 1%) and durations (1 to 24 hours) revealed that 0.5% TZ consistently yielded 100% viability across all durations, making it the most effective treatment. Statistical analysis confirmed that TZ concentration, staining duration, and their interaction significantly influenced viability outcomes (p < 0.001). The seeds exhibited a clear triphasic water imbibition pattern, with fresh weight increasing by 158.79% at 30 hours before plateauing, indicative of typical orthodox seed hydration. Germination trials demonstrated that no pre-treatment was necessary, as seeds showed high viability without signs of physical or physiological dormancy. Desiccation trials, followed by storage at –20 °C, further confirmed the orthodox nature of the seeds. Germination improved from 89.26% in fresh seeds to 90.83% after desiccation and 95.38% after three months of cold storage. These results establish G. sylvestre seeds as viable, desiccation-tolerant, and suitable for direct sowing and long-term conservation under low-temperature storage conditions.