Molecular Characterization of Mulberry Germplasm through ISSR Markers
Aruna N. *
Department of Sericulture, Forest College and Research Institute, Mettupalayam, Tamil Nadu, India.
Mavilashaw V P.
Department of Agriculture Entomology, The Indian Agriculture College, Radhapuram, Tirunelveli, Tamil Nadu, India.
Saranya, M.
Department of Entomology, Adhiparasakthi Agricultural College, Kalavai, India.
Krishnamoorthy S V.
Department of Sericulture, Forest College and Research Institute, Mettupalayam, Tamil Nadu, India.
Parthiban K T
Forest College and Research Institute, Mettupalayam, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Inter simple sequence repeat (ISSR)-PCR is a technique, which involves the use of microsatellite sequence as primers in a polymerase chain reaction to generate multilocus markers. Twenty-four mulberry accessions were obtained from the Central Sericulture Germplasm Resource Center, Hosur, Tamil Nadu were screened using Inter simple sequence repeat technique, with eight primers. Out of eight primers, only three primers viz., UBC 807, UBC 809 and UBC 811 produced 100 per cent polymorphism in mulberry accessions. The highest Polymorphism information content value was observed with the primer UBC 807 and the lowest PIC value was observed with the primer UBC 811. The mulberry accessions A18 (MI-0685) and A8 (MI-0535) were found to be the closest at the similarity coefficient of 0.93. Mean genetic diversity and PIC were 0.35 to 0.29, respectively. The genetic similarity coefficient ranged from 0.913 A18 (MI-0685) to 0.535 A8 (MI-0535). The phylogenetic tree was obtained using the UPGMA method using the total number of amplified Inter simple sequence repeat fragments. The result from the cluster analysis indicated that the mulberry accessions MI-0017, MI-0763, MI-0663, MI-0652, MI-0349, V-1 (check variety), MI-0034 and MI-0768 were distinct from other accessions.
Keywords: Mulberry, molecular marker, genetic diversity, inter simple sequence repeat