Artificial Intelligence in the Assessment of Seed Physiological Quality: A Critical Review of Spectral, Imaging and Learning-based Approaches for Digital Seed Technology

Mônica Araújo de Souza Santos *

Department of Agricultural Sciences, State University of Montes Claros (UNIMONTES), Janaúba Campus, Janaúba, MG, Brazil.

Andréia Márcia Santos de Souza David

Department of Agricultural Sciences, State University of Montes Claros (UNIMONTES), Janaúba Campus, Janaúba, MG, Brazil.

João Rafael Prudêncio dos Santos

Department of Education, Federal Institute of Northern Minas Gerais, Campus Araçuaí, Araçuaí, MG, Brazil.

Anne Karolina de Melo Souza

Department of Agricultural Sciences, State University of Montes Claros (UNIMONTES), Janaúba Campus, Janaúba, MG, Brazil.

Rodrigo Silva Barbosa

Department of Agricultural Sciences, State University of Montes Claros (UNIMONTES), Janaúba Campus, Janaúba, MG, Brazil.

Denner Junio Ramos Xavier

Department of Agricultural Sciences, State University of Montes Claros (UNIMONTES), Janaúba Campus, Janaúba, MG, Brazil.

Samanta Saira Souza

Department of Education, Federal Institute of Northern Minas Gerais, Campus Porteirinha, Porteirinha, MG, Brazil.

*Author to whom correspondence should be addressed.


Abstract

Seed physiological quality, expressed through viability, germination capacity and vigour, determines stand establishment, uniformity and yield potential, yet the reference tests that define it remain destructive, labour-intensive and slow. Over the past decade, artificial intelligence, encompassing classical machine learning and deep learning applied to spectral and image data, has been advanced as a rapid, non-destructive alternative. This review critically evaluates that body of work and asks how far learning-based methods have genuinely improved on established seed testing rather than merely reproduced it under favourable conditions. Evidence was drawn from peer-reviewed studies applying near-infrared spectroscopy, hyperspectral and multispectral imaging, red-green-blue and X-ray imaging, and automated seedling analysis to viability, vigour and germination endpoints across cereal, oilseed, vegetable and tree species. The literature demonstrates consistently high classification and prediction performance under controlled, single-laboratory conditions, with hyperspectral imaging combined with variable selection and convolutional networks emerging as the dominant paradigm for viability and vigour. Deep learning frequently, though not invariably, outperforms chemometric baselines, and transfer learning, data augmentation and generative modelling have been introduced to counter the field's most persistent constraint, namely small and imbalanced datasets. Recurrent weaknesses temper these results: reliance on artificial ageing as a proxy for natural deterioration, narrow cultivar and environmental coverage, scarce external validation, inconsistent reference labelling, and limited interpretability. Reported accuracy therefore reflects internal discrimination more than demonstrated field relevance, and comparability across studies is undermined by heterogeneous endpoints and the absence of shared benchmarks. Progress toward operational digital seed technology will depend less on novel architectures than on representative multi-season datasets, standardised protocols, biologically grounded reference labels and prospective validation against germination and field emergence. The synthesis distinguishes well-supported conclusions from preliminary claims and sets out prioritised research directions to move the field from proof of concept toward dependable, transferable decision support.

Keywords: Seed vigour, seed viability, hyperspectral imaging, deep learning, near-infrared spectroscopy, non-destructive testing, germination prediction, digital agriculture


How to Cite

Santos, Mônica Araújo de Souza, Andréia Márcia Santos de Souza David, João Rafael Prudêncio dos Santos, Anne Karolina de Melo Souza, Rodrigo Silva Barbosa, Denner Junio Ramos Xavier, and Samanta Saira Souza. 2026. “Artificial Intelligence in the Assessment of Seed Physiological Quality: A Critical Review of Spectral, Imaging and Learning-Based Approaches for Digital Seed Technology”. International Journal of Plant & Soil Science 38 (8):700-721. https://doi.org/10.9734/ijpss/2026/v38i86253.

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