Impact of Two Herbicides (Zoom and Sencorate) on Physiological Parameters (Chlorophyll, Proline and Total Protein), Enzymatic (CAT and APX) and Non-Enzymatic Biomarkers (MDA and GSH) of the Species Orthotrichum affine
Khouloud Boukehili
Laboratory of Biochemistry, Department of Biology, Faculty of Natural Sciences and Life, University of Mohamed Cherif Messaadia, PBOX/1553, 41000, SoukAhras, Algeria.
Fadila Khaldi *
Laboratory of Biochemistry, Department of Biology, Faculty of Natural Sciences and Life, University of Mohamed Cherif Messaadia, PBOX/1553, 41000, SoukAhras, Algeria.
Meriem Kharrachi
Laboratory of Biochemistry, Department of Biology, Faculty of Natural Sciences and Life, University of Mohamed Cherif Messaadia, PBOX/1553, 41000, SoukAhras, Algeria.
*Author to whom correspondence should be addressed.
Abstract
The bryophytes of the genus Orthotrichum (O. affine) are treated in hydroponic conditions with 125, 250, 500, 1000 and 1500 mg/L of two herbicides (Zoom and Sencorate) for 3, 7 and 14 days. The signs of toxicity are manifested by disturbances in chlorophyll contents (a, b and a + b), accompanied by an increase of the content of proline (3 and 7 days) and total protein. Our results also showed a decrease in the GSH level with an increased rate of proline. Regarding the CAT and APX, we showed low activity of these enzymes seems insignificant for the majority of time reflecting the high tolerance of this species to pollutants.
Keywords: Orthotricum affine, chlorophyll, proline, protein, enzymatic activity, biomarkers, sencorate, zoom