A Comparative Study of β-galactosidase and Alanine Amino Transferase Enzymes during the Germination of Rice, Wheat, Ragi and Bajra
Chandra Kumari
Department of Biochemistry, JSS College of Arts, Commerce and Science, Ooty Road, Mysore, Karnataka, India.
Savitha Gujjaiah *
Department of Chemistry, Maharani’s Science College for Women, Mysore, Karnataka, India.
*Author to whom correspondence should be addressed.
Abstract
Biomolecules are converted by the action of enzymes during the germination process and these are translocated to the growing seedlings serving as an enzyme source and for the synthesis of new biomolecules. The objective in the present study was to quantify the enzymatic activity of β-galactosidase and alanine amino transferase in rice, wheat, ragi and bajra on different days of germination. Germination was carried out for a period of 8 days. The enzymes activity increased differentially to an extent on the different days on germination in rice, wheat, ragi and bajra. This indicates the breakdown of molecules differs and the mechanism which is operating during the germination varies from species to species, depending on the requirement for the developing seedlings.
Keywords: Germinating seedlings, alanine amino transferase, β-galactosidase, rice, wheat, ragi and bajra.