Isolation and Characterization of Phosphate Solubilizing Bacteria from Calcareous Soil
S. D. Gaikwad
*
Department of Plant Pathology and Agricultural Microbiology, College of Agriculture, Pune, Mahatma Phule Krishi Vidyapeeth, Maharashtra, India.
P. A. Bhosale
Department of Plant Pathology and Agricultural Microbiology, College of Agriculture, Pune, Mahatma Phule Krishi Vidyapeeth, Maharashtra, India.
P. V. Ukey
ICAR National Research center for Grapes Pune, Maharashtra, India.
K. B. landage
Biological Nitrogen Fixation Scheme, College of Agriculture Pune 05, Mahatma Phule Krishi Vidyapeeth, Rahuri. Maharashtra, India.
*Author to whom correspondence should be addressed.
Abstract
The present investigation was conducted with aim to isolate and characterize phosphate solubilizing bacteria from calcareous soil. Four efficient PSB isolates were obtained from fourteen soil samples collected from different locations. Based on morphological and biochemical characterization, highly phosphate solubilizing isolates were identified as Pseudomonas fluorescence, Bacillus subtilis, Bacillus thuringiensis and Pseudomonas aeruginosa respectively. The amount of Pi released from TCP by the isolates at 10 DAI ranged from 23.2 to 30.5 % and zone of solubilization recorded between 6 -13 mm. Among them PSB isolate 1 (Pseudomonas fluorescence) recorded highest Pi released from TCP broth i:e (30.5 %) and zone of solubilization recorded (13 mm) than the other isolates tested.
Keywords: Isolation, pseudomonas fluorescence, bacillus subtilis, bacillus thuringiensis, pseudomonas aeruginosa, phosphorus fixation, calcareous soil.